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AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9*
4'-(Aminomethyl)fluorescein is an excellent building block for developing fluoresceinated bioconjugates. Its amino group readily reacts with activated carboxy esters (such as NHS ester).
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 216.193 µL | 1.081 mL | 2.162 mL | 10.81 mL | 21.619 mL |
| 5 mM | 43.239 µL | 216.193 µL | 432.386 µL | 2.162 mL | 4.324 mL |
| 10 mM | 21.619 µL | 108.096 µL | 216.193 µL | 1.081 mL | 2.162 mL |
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Citations
View all 2 citations: Citation Explorer
Targeted cross-linker delivery for the in situ mapping of protein conformations and interactions in mitochondria
Authors: Chen, Yuwan and Zhou, Wen and Xia, Yufei and Zhang, Weijie and Zhao, Qun and Li, Xinwei and Gao, Hang and Liang, Zhen and Ma, Guanghui and Yang, Kaiguang and others,
Journal: Nature Communications (2023): 3882
Authors: Chen, Yuwan and Zhou, Wen and Xia, Yufei and Zhang, Weijie and Zhao, Qun and Li, Xinwei and Gao, Hang and Liang, Zhen and Ma, Guanghui and Yang, Kaiguang and others,
Journal: Nature Communications (2023): 3882
Investigation of the Small Size of Nanobodies for a Sensitive Fluorescence Polarization Immunoassay for Small Molecules: 3-Phenoxybenzoic Acid, an Exposure Biomarker of Pyrethroid Insecticides as a Model
Authors: Wang, Yulong and Li, Zhenfeng and Barnych, Bogdan and Huo, Jingqian and Wan, Debin and Vasylieva, Natalia and Xu, Junli and Li, Pan and Liu, Beibei and Zhang, Cunzheng and others,
Journal: Journal of agricultural and food chemistry (2019): 11536--11541
Authors: Wang, Yulong and Li, Zhenfeng and Barnych, Bogdan and Huo, Jingqian and Wan, Debin and Vasylieva, Natalia and Xu, Junli and Li, Pan and Liu, Beibei and Zhang, Cunzheng and others,
Journal: Journal of agricultural and food chemistry (2019): 11536--11541
References
View all 15 references: Citation Explorer
Type and location of fluorescent probes incorporated into the potent mu-opioid peptide [Dmt]DALDA affect potency, receptor selectivity and intrinsic efficacy
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Authors: Schiller PW, Berezowska I, Weltrowska G, Chen H, Lemieux C, Chung NN.
Journal: J Pept Res (2005): 556
Assessment of S-nitrosothiols on diaminofluorescein gels
Authors: King M, Gildemeister O, Gaston B, Mannick JB.
Journal: Anal Biochem (2005): 69
Authors: King M, Gildemeister O, Gaston B, Mannick JB.
Journal: Anal Biochem (2005): 69
Detection of polychlorinated biphenyls using an antibody column in tandem with a fluorescent liposome column. Effect of albumin on phospholipase A2-catalyzed membrane leakage
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Journal: J Chromatogr A (2005): 229
Authors: Liu XY, Nakamura C, Nakamura N, Hirano T, Shinbo T, Miyake J.
Journal: J Chromatogr A (2005): 229
Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery
Authors: Dubruel P, Christiaens B, Rosseneu M, V and ekerckhove J, Grooten J, Goossens V, Schacht E.
Journal: Biomacromolecules (2004): 379
Authors: Dubruel P, Christiaens B, Rosseneu M, V and ekerckhove J, Grooten J, Goossens V, Schacht E.
Journal: Biomacromolecules (2004): 379
Effect of glyphosate on the microbial activity of two Brazilian soils
Authors: Araujo AS, Monteiro RT, Abarkeli RB.
Journal: Chemosphere (2003): 799
Authors: Araujo AS, Monteiro RT, Abarkeli RB.
Journal: Chemosphere (2003): 799
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