ABY Dye qPCR Calibration Solution 10,000X

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as ABY, will depend on the device used. The AAT Bioquest ABY dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.

Prepare a ABY calibration plate:

Prepare a ABY Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X ABY Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.

Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the ABY Dye qPCR Calibration working solution to all wells of a reaction plate.

Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For complete calibration instructions, refer to the maintenance guide for your instrument.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
VIC Dye qPCR Calibration Solution 10,000X	526	543	-
NED Dye qPCR Calibration Solution 10,000X	545	567	74000¹
FAM Dye qPCR Calibration Solution 10,000X	493	517	83000
Cy5 Dye qPCR Calibration Solution 10,000X	651	670	250000¹
ROX Dye qPCR Calibration Solution 10,000X	578	595	-
SYBR Dye qPCR Calibration Solution 10,000X	498	522	-
TAMRA Dye qPCR Calibration Solution 10,000X	552	578	90000
JUN Dye qPCR Calibration Solution 10,000X	610	628	110000¹
MP650 Dye qPCR Calibration Solution 10,000X	639	653	250000¹
ABY Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	559	570	120000¹
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References

View all 50 references: Citation Explorer

Bocaparvovirus in infants hospitalized in a neonatal intensive care unit in Midwest Brazil during the COVID-19 pandemic (2021-2022).
Authors: de Faria Gonçalves, Aline and Anjos, Déborah and de Oliveira Peixoto, Fernanda Aparecida and Franco, Fernanda Craveiro and Silva-Sales, Marcelle and Fiaccadori, Fabíola Souza and Porto, Pedro Soares and Souza, Menira
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2024): 315-322

Evaluation of Extraction Methods to Detect Noroviruses in Ready-to-Eat Raw Milk Minas Artisanal Cheese.
Authors: Cantelli, Carina Pacheco and Silva, Marcio Roberto and Pimenta, Laís Marques and Tavares, Guilherme Caetano Lanzieri and Baduy, Gabriel Assad and Duch, André Almeida Santos and Menezes, Liliane Denize Miranda and Fialho, Alexandre Madi and Maranhão, Adriana Gonçalves and Fumian, Tulio Machado and Miagostovich, Marize Pereira and Leite, José Paulo Gagliardi
Journal: Food and environmental virology (2024)

Association of the rs1126616 and rs9138 Variants in the SPP1 Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis.
Authors: Rivera-Cameras, Alicia and Gallegos-Arreola, Martha Patricia and Morán-Moguel, María Cristina and Salazar-Páramo, Mario and Alcaraz-López, Miriam Fabiola and Echeverría-González, Gustavo and Topete-Reyes, Jorge Fernando and Franco-Chávez, Sergio Adalberto and Dávalos-Rodríguez, Ingrid Patricia
Journal: International journal of molecular sciences (2024)

A phase 2a open-label clinical trial to determine the effect of famciclovir on EBV activity as measured by EBV shedding in the saliva of patients with multiple sclerosis.
Authors: Dobson, Ruth and Holden, David and Vickaryous, Nicola and Bestwick, Jonathan and George, Katila and Sayali, Tatiana and Bianchi, Lucia and Wafa, Mohammad and Gold, Julian and Giovannoni, Gavin
Journal: Multiple sclerosis (Houndmills, Basingstoke, England) (2024): 63-70

Species composition, infection rate and detection of resistant alleles in Anopheles funestus (Diptera: Culicidae) from Lare, a malaria hotspot district of Ethiopia.
Authors: Woyessa, Delelegn and Morou, Evangelia and Wipf, Nadja and Dada, Nsa and Mavridis, Konstantinos and Vontas, John and Yewhalaw, Delenasaw
Journal: Malaria journal (2023): 233

Page updated on November 15, 2024

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Catalog Number	67037
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Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	559
Emission (nm)	570
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501