Streptavidin Magnetic Beads
3 μm, 1.0%
Streptavidin magnetic beads capture biotinylated biomolecules, enabling separation of antibodies, proteins, antigens, nucleic acids, cells and more.
- High binding capacity — greater than 3000 pmol of free biotin per mg of streptavidin magnetic beads
- Low nonspecific binding — pre-blocked beads allow clean purification, even of complex samples
- Consistent performance — monodispersed particles manufactured to ensure minimal batch-to-batch variation
- Experimental convenience — fast binding kinetics simplifies purification process against conventional methods such as columns or centrifugation and permits HTS applications
These streptavidin-coated magnetic beads are uniform particles and are available in the following diameters: 0.6 µm, 1 µm and 3 µm. Magnetic beads are provided in a 1% (10 mg / mL) aqueous solution with additives [1].
The surface of the magnetic beads are covalently coated with streptavidin protein. The specificity and strength of the streptavidin-biotin interaction is well established, with each streptavidin tetramer capable of binding four biotin molecules, with a dissociation constant (Kd) of approximately 10-14 mol/L [2]. This allows for fast and specific capture of targets labeled with biotin, such as oligonucleotides, peptides, proteins and antibodies.
The usage of magnetic beads greatly streamlines the purification of crude samples. Simply incubate with target analytes, retain beads using a magnet, while washing unbound contaminates from the sample tube. An elution buffer can subsequently be used to recover captured biomolecules. Compared to other methods of purification, streptavidin magnetic beads can be seamlessly integrated into automatic sample preparation systems and high-throughput screening (HTS) applications.
Product applications
- Purification of biotinylated biomolecules
- Pull-down assays to study protein-protein and DNA-protein interactions
- Extraction of biotin-labeled PCR products
- Immunoprecipitation of complex samples
[1] Storage buffer contains 10 mM Tris (pH 7.4), 0.1% BSA, 0.05% Tween-20 and 0.1% ProClin 300.
[2] https://www.aatbio.com/catalog/biotin-and-streptavidin
[2] https://www.aatbio.com/catalog/biotin-and-streptavidin
References
View all 50 references: Citation Explorer
Soluble expression and immunogenicity analysis of capsid proteins of porcine circoviruses types 2, 3, and 4.
Authors: Zhang, Huimin and Li, Xue and Lv, Xinru and Han, Yaqi and Zheng, Jiawei and Ren, Linzhu
Journal: Veterinary journal (London, England : 1997) (2024): 106199
Authors: Zhang, Huimin and Li, Xue and Lv, Xinru and Han, Yaqi and Zheng, Jiawei and Ren, Linzhu
Journal: Veterinary journal (London, England : 1997) (2024): 106199
Biotinylated cyclic naphthalene diimide as a searching tool for G4 sites on the genome.
Authors: Fujii, Satoshi and Sato, Shinobu and Hidaka, Ryuki and Takenaka, Shigeori
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2024): 943-950
Authors: Fujii, Satoshi and Sato, Shinobu and Hidaka, Ryuki and Takenaka, Shigeori
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2024): 943-950
Assessing MAPPs assay as a tool to predict the immunogenicity potential of protein therapeutics.
Authors: Di Ianni, Andrea and Fraone, Tiziana and Balestra, Piercesare and Cowan, Kyra and Riccardi Sirtori, Federico and Barbero, Luca
Journal: Life science alliance (2024)
Authors: Di Ianni, Andrea and Fraone, Tiziana and Balestra, Piercesare and Cowan, Kyra and Riccardi Sirtori, Federico and Barbero, Luca
Journal: Life science alliance (2024)
Magnetic Bead-Based Workflow for Sensitive and Streamlined Cell Surface Proteomics.
Authors: Dieters-Castator, Dylan Z and Manzanillo, Paolo and Yang, Han-Yin and Modak, Rucha V and Rardin, Matthew J and Gibson, Bradford W
Journal: Journal of proteome research (2024): 618-632
Authors: Dieters-Castator, Dylan Z and Manzanillo, Paolo and Yang, Han-Yin and Modak, Rucha V and Rardin, Matthew J and Gibson, Bradford W
Journal: Journal of proteome research (2024): 618-632
Screening and identification of peptidyl arginine deiminase 4 inhibitors from herbal plants extracts and purified natural products by a trypsin assisted sensitive immunoassay based on streptavidin magnetic beads.
Authors: Zhao, Juanjuan and Zhang, Shengxiang and Dong, Jianhui and Chen, Xufei and Zuo, Haiyue and Li, Yanfeng and Gao, Chunli and Zhao, Zeyuan and Qiu, Xingtai and Tang, Zichao and Deng, Nan and Zhao, Weining and Ou, Junjie and Bian, Yangyang
Journal: Talanta (2024): 126611
Authors: Zhao, Juanjuan and Zhang, Shengxiang and Dong, Jianhui and Chen, Xufei and Zuo, Haiyue and Li, Yanfeng and Gao, Chunli and Zhao, Zeyuan and Qiu, Xingtai and Tang, Zichao and Deng, Nan and Zhao, Weining and Ou, Junjie and Bian, Yangyang
Journal: Talanta (2024): 126611
Page updated on August 9, 2024