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8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6*

8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] is the cell-impermeable fluorescent pH indicator used for slightly acidic pH range (pKa = 6.4). It is highly water-soluble.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM190.701 µL953.507 µL1.907 mL9.535 mL19.07 mL
5 mM38.14 µL190.701 µL381.403 µL1.907 mL3.814 mL
10 mM19.07 µL95.351 µL190.701 µL953.507 µL1.907 mL

Molarity calculator

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Spectrum

Citations

View all 4 citations: Citation Explorer
Two-Photon Image-Guided Automated 3D Locating of Micropipette Tip
Authors: Li, Zizhen and Li, Jie and Zhao, Qili and Zhao, Xin and Sun, Mingzhu
Journal: (2024): 4469--4474
Comparative responses of two maize genotypes with contrasting drought tolerance to biochar application
Authors: Ruan, Renjie and Lambers, Hans and Wang, Yaosheng
Journal: Biochar (2024): 1--15
Effects of biochar amendment on root growth and plant water status depend on maize genotypes
Authors: Ruan, Renjie and Wang, Yaosheng
Journal: Agricultural Water Management (2024): 108688
Method for Passive Droplet Sorting after Photo-Tagging
Authors: Dobson, Chandler and Zielke, Claudia and Pan, Ching W and Feit, Cameron and Abbyad, Paul
Journal: Micromachines (2020): 964

References

View all 20 references: Citation Explorer
Temperature dependence of excited-state proton transfer in water electrolyte solutions and water-methanol solutions
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9039
Emission-based optical carbon dioxide sensing with HPTS in green chemistry reagents: room-temperature ionic liquids
Authors: Oter O, Ertekin K, Topkaya D, Alp S.
Journal: Anal Bioanal Chem (2006): 1225
The effect of neuronal morphology and membrane-permeant weak acid and base on the dissipation of depolarization-induced pH gradients in snail neurons
Authors: Pantazis A, Keegan P, Postma M, Schwiening CJ.
Journal: Pflugers Arch (2006): 175
Effect of electrolytes on the excited-state proton transfer and geminate recombination
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5573
Universal behaviour of gel formation from acrylamide-carrageenan mixture around the gel point: a fluorescence study
Authors: Aktas DK, Evingur GA, Pekcan O.
Journal: J Biomol Struct Dyn (2006): 83
Page updated on December 17, 2024

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Physical properties

Molecular weight

524.38

Solvent

Water

Spectral properties

Excitation (nm)

452

Emission (nm)

512

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

6358-69-6
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