iFluor® Ultra 594 succinimidyl ester

iFluor® Ultra 594 succinimidyl ester is an amine-reactive, red-fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence imaging and flow cytometry applications.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 586/600 nm.
Enhanced Brightness: Produces significantly brighter antibody conjugates than Alexa Fluor® 594 under identical conditions.
pH Stability: Maintains pH-independent fluorescence from pH 4 to 10.

HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> Ultra 594 goat anti-mouse IgG conjugate or Alexa Fluor<sup>®</sup> 594 goat anti-mouse IgG.

Protocol

Catalog	Size	Price
71650	1 mg	Price
71651	100 ug	Price
71652	5 mg	Price

Overview

Fluorescent dye-conjugated antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, flow cytometry, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled antibody include higher sensitivity, multiplexing capabilities, and ease of use. iFluor® Ultra family is a recent upgrade of our popular iFluor® dyes and optimized for labeling antibodies used for fluorescence imaging and flow cytometry applications. Antibody conjugates prepared with iFluor® Ultra 594 are far superior to the conjugates of other existing similar dyes such as Alexa Fluor® 594. iFluor® Ultra 594 conjugates are significantly brighter than the conjugates prepared with Alexa Fluor® 594 under the same conditions. Additionally, the fluorescence of iFluor® Ultra 594 is not affected by pH (4-10). iFluor® Ultra 594 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® Ultra 594 has spectral properties and reactivity similar to Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® Ultra 594 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	69.603 µL	348.015 µL	696.03 µL	3.48 mL	6.96 mL
5 mM	13.921 µL	69.603 µL	139.206 µL	696.03 µL	1.392 mL
10 mM	6.96 µL	34.801 µL	69.603 µL	348.015 µL	696.03 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1436.72 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

MicroRNA-126 inhibits pathological retinal neovascularization via suppressing vascular endothelial growth factor expression in a rat model of retinopathy of prematurity.

Authors:

Fan, Yuan-Yao and Liu, Chi-Hsien and Wu, An-Lun and Chen, Hung-Chi and Hsueh, Yi-Jen and Chen, Kuan-Jen and Lai, Chi-Chun and Huang, Chung-Ying and Wu, Wei-Chi

Journal:

European journal of pharmacology (2021): 174035

Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.

Authors:

Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R

Journal:

Brain structure & function (2021)

A fully integrated isotachophoresis with a programmable microfluidic platform.

Authors:

Shebindu, Adam and Somaweera, Himali and Estlack, Zachary and Kim, Jungtae and Kim, Jungkyu

Journal:

Talanta (2021): 122039

Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides.

Authors:

Iwata, Takahiro and Hirose, Hisaaki and Sakamoto, Kentarou and Hirai, Yusuke and Arafiles, Jan Vincent V and Akishiba, Misao and Imanishi, Miki and Futaki, Shiroh

Journal:

Angewandte Chemie (International ed. in English) (2021)

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).

Authors:

Seo, Hyesu and Kim, Kyunghee and Park, Woong June

Journal:

Biomolecules (2021)

Physical properties

Molecular weight	1436.72
Solvent	DMSO

Spectral properties

Absorbance (nm)	585
Correction factor (260 nm)	0.07
Correction factor (280 nm)	0.05
Extinction coefficient (cm ^-1 M ^-1)	180000 ¹
Excitation (nm)	586
Emission (nm)	600
Quantum yield	0.52 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 31, 2026