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ReadiCleave™ ROXtra SSL-NHS ester
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ ROXtra succinimidyl ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the ROXtra fluorophore from the target molecule. ROXtra has almost identical spectra of ROX with higher fluorescence quantum yield and improved stability. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| ReadiCleave™ Cy5-SSL-NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 |
| ReadiCleave™ XFD532 SSL-NHS ester | 534 | 553 | 81000 | 0.611 | 0.09 |
| ReadiCleave™ FAM SSL-NHS ester | 491 | 516 | 73000 | 0.92 | 0.35 |
References
View all 42 references: Citation Explorer
Developmental Validation of a Rapidly Mutating Y-STR Panel Labeled by Six Fluoresceins for Forensic Research.
Authors: Jin, Xiaoye and Zhang, Hongling and Ren, Zheng and Wang, Qiyan and Liu, Yubo and Ji, Jingyan and Zhang, Han and Yang, Meiqing and Zhou, Yongsong and Huang, Jiang
Journal: Frontiers in genetics (2022): 777440
Authors: Jin, Xiaoye and Zhang, Hongling and Ren, Zheng and Wang, Qiyan and Liu, Yubo and Ji, Jingyan and Zhang, Han and Yang, Meiqing and Zhou, Yongsong and Huang, Jiang
Journal: Frontiers in genetics (2022): 777440
An aptasensor for the detection of Pb2+ based on photoinduced electron transfer between a G-quadruplex-hemin complex and a fluorophore.
Authors: Wang, Ya and Ye, Tai and Yuan, Min and Cao, Hui and Yu, Jingsong and Yin, Fengqin and Wu, Xiuxiu and Hao, Liling and Xu, Fei
Journal: Luminescence : the journal of biological and chemical luminescence (2022): 14-20
Authors: Wang, Ya and Ye, Tai and Yuan, Min and Cao, Hui and Yu, Jingsong and Yin, Fengqin and Wu, Xiuxiu and Hao, Liling and Xu, Fei
Journal: Luminescence : the journal of biological and chemical luminescence (2022): 14-20
Bimetallic organic framework-based aptamer sensors: a new platform for fluorescence detection of chloramphenicol.
Authors: Lu, Zijing and Jiang, Yansong and Wang, Peng and Xiong, Weiwei and Qi, Baoping and Zhang, Yingkun and Xiang, Dongshan and Zhai, Kun
Journal: Analytical and bioanalytical chemistry (2020): 5273-5281
Authors: Lu, Zijing and Jiang, Yansong and Wang, Peng and Xiong, Weiwei and Qi, Baoping and Zhang, Yingkun and Xiang, Dongshan and Zhai, Kun
Journal: Analytical and bioanalytical chemistry (2020): 5273-5281
In Situ Imaging of Pathological Collagen by Electrostatic Repulsion-Destabilized Peptide Probes.
Authors: Cai, Xiangdong and Wei, Wenyu and Liu, Zhao and Bai, Zhongtian and Lei, Junqiang and Xiao, Jianxi
Journal: ACS applied bio materials (2020): 7492-7499
Authors: Cai, Xiangdong and Wei, Wenyu and Liu, Zhao and Bai, Zhongtian and Lei, Junqiang and Xiao, Jianxi
Journal: ACS applied bio materials (2020): 7492-7499
SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
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