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7-Hydroxycoumarin-4-acetic acid, succinimidyl ester
7-Hydroxycoumarin-4-acetic acid is used for labeling peptides and proteins to give blue fluorescence. It can also be used for labeling other biological molecules.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-4-acetic acid, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 315.209 µL | 1.576 mL | 3.152 mL | 15.76 mL | 31.521 mL |
| 5 mM | 63.042 µL | 315.209 µL | 630.418 µL | 3.152 mL | 6.304 mL |
| 10 mM | 31.521 µL | 157.604 µL | 315.209 µL | 1.576 mL | 3.152 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 5 references: Citation Explorer
Stopped-flow kinetic analysis of long-chain fatty acid dissociation from bovine serum albumin
Authors: Demant EJ, Richieri GV, Kleinfeld AM.
Journal: Biochem J (2002): 809
Authors: Demant EJ, Richieri GV, Kleinfeld AM.
Journal: Biochem J (2002): 809
Continuous recording of long-chain acyl-coenzyme a synthetase activity using fluorescently labeled bovine serum albumin
Authors: Demant EJ, Nystrom BT.
Journal: Anal Biochem (2001): 38
Authors: Demant EJ, Nystrom BT.
Journal: Anal Biochem (2001): 38
Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the fluorometric measurement of fatty acid concentrations
Authors: Demant EJ., undefined
Journal: Anal Biochem (1999): 366
Authors: Demant EJ., undefined
Journal: Anal Biochem (1999): 366
Covalent complexes between serum albumin and 7-hydroxycoumarin-4-acetic acid: synthesis and applications in the spectrophotometric detection of long-chain fatty acids
Authors: Demant EJ., undefined
Journal: Biochim Biophys Acta (1996): 43
Authors: Demant EJ., undefined
Journal: Biochim Biophys Acta (1996): 43
Structure-activity relationship of coumarins in xanthine oxidase inhibition
Authors: Chang WS, Chiang HC.
Journal: Anticancer Res (1995): 1969
Authors: Chang WS, Chiang HC.
Journal: Anticancer Res (1995): 1969
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