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7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester

7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is an amine-reactive blue fluorophore that has pH-dependent and environment-sensitive fluorescence. It is widely used for preparing bioconjugates of blue fluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM301.859 µL1.509 mL3.019 mL15.093 mL30.186 mL
5 mM60.372 µL301.859 µL603.719 µL3.019 mL6.037 mL
10 mM30.186 µL150.93 µL301.859 µL1.509 mL3.019 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References

View all 1 references: Citation Explorer
Sensitivity enhancement of fluorescent pH indicators using pH-dependent energy transfer
Authors: Gabor G, et al.
Journal: Anal Chim Acta (1995): 131
Page updated on December 17, 2024

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Physical properties

Molecular weight

331.28

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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