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7-Hydroxy-4-methylcoumarin-3-acetic acid *CAS 5852-10-8*
7-Hydroxy-4-methylcoumarin-3-acetic acid is a blue fluorophore that has pH-dependent and environment-sensitive fluorescence. This coumarin is increasingly used to label peptides, nucleotides and carbohydrates.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin-3-acetic acid *CAS 5852-10-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 426.967 µL | 2.135 mL | 4.27 mL | 21.348 mL | 42.697 mL |
| 5 mM | 85.393 µL | 426.967 µL | 853.935 µL | 4.27 mL | 8.539 mL |
| 10 mM | 42.697 µL | 213.484 µL | 426.967 µL | 2.135 mL | 4.27 mL |
Molarity calculator
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References
View all 1 references: Citation Explorer
Sensitivity enhancement of fluorescent pH indicators using pH-dependent energy transfer
Authors: Gabor G, et al.
Journal: Anal Chim Acta (1995): 131
Authors: Gabor G, et al.
Journal: Anal Chim Acta (1995): 131
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