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6-VIC phosphoramidite
6-VIC phosphoramidite is an asymmetrical xanthene dye widely used to prepare fluorescence-labeled oligonucleotides and design qPCR probes, such as TaqMan, Molecular Beacon, and Scorpion. Its spectral characteristics are similar to HEX and JOE, emitting fluorescence in the green-yellow range. 6-VIC phosphoramidite can efficiently label oligonucleotides at their 5'-end using standard or automated synthesis protocols. Protecting groups on the 6-VIC moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| 6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0* | 493 | 517 | 83000 |
| 6-Fluorescein phosphoramidite | 498 | 517 | 800001 |
| 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6* | 521 | 542 | 73000 |
| 6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite] | 533 | 559 | 73000 |
| 6-VIC, SE [6-VIC NHS ester] | 526 | 543 | - |
| 6-JOE Phosphoramidite | 520 | 545 | 750001 |
| 5-VIC phosphoramidite | 526 | 543 | - |
| 6-VIC Azide | 526 | 543 | - |
| 6-VIC Alkyne | 526 | 543 | - |
References
View all 50 references: Citation Explorer
Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing.
Authors: Zhang, Yanjun and Tang, Yin and Sun, Zhongxing and Jia, Junqi and Fang, Yuan and Wan, Xinyi and Fang, Dong
Journal: Genome research (2023): 412-426
Authors: Zhang, Yanjun and Tang, Yin and Sun, Zhongxing and Jia, Junqi and Fang, Yuan and Wan, Xinyi and Fang, Dong
Journal: Genome research (2023): 412-426
CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing.
Authors: Carbonell-Sala, Silvia and Lagarde, Julien and Nishiyori, Hiromi and Palumbo, Emilio and Arnan, Carme and Takahashi, Hazuki and Carninci, Piero and Uszczynska-Ratajczak, Barbara and Guigó, Roderic
Journal: bioRxiv : the preprint server for biology (2023)
Authors: Carbonell-Sala, Silvia and Lagarde, Julien and Nishiyori, Hiromi and Palumbo, Emilio and Arnan, Carme and Takahashi, Hazuki and Carninci, Piero and Uszczynska-Ratajczak, Barbara and Guigó, Roderic
Journal: bioRxiv : the preprint server for biology (2023)
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing.
Authors: Ge, Yang and van Roon, Lieke and Chen, H Sophia and Methorst, Ruben and Paton, Martin and DeRuiter, Marco C and Kielbasa, Szymon M and Jongbloed, Monique R M
Journal: Journal of visualized experiments : JoVE (2022)
Authors: Ge, Yang and van Roon, Lieke and Chen, H Sophia and Methorst, Ruben and Paton, Martin and DeRuiter, Marco C and Kielbasa, Szymon M and Jongbloed, Monique R M
Journal: Journal of visualized experiments : JoVE (2022)
Multiplexed Single-Nucleus RNA Sequencing Using Lipid-Oligo Barcodes.
Authors: Zhang, Qi and Kim, Seong Won and Gorham, Joshua M and DeLaughter, Daniel M and Ward, Tarsha and Seidman, Christine E and Seidman, Jonathan G
Journal: Current protocols (2022): e579
Authors: Zhang, Qi and Kim, Seong Won and Gorham, Joshua M and DeLaughter, Daniel M and Ward, Tarsha and Seidman, Christine E and Seidman, Jonathan G
Journal: Current protocols (2022): e579
High-Throughput Activity Profiling of RNA-Cleaving DNA Catalysts by Deoxyribozyme Sequencing (DZ-seq).
Authors: Sednev, Maksim V and Liaqat, Anam and Höbartner, Claudia
Journal: Journal of the American Chemical Society (2022): 2090-2094
Authors: Sednev, Maksim V and Liaqat, Anam and Höbartner, Claudia
Journal: Journal of the American Chemical Society (2022): 2090-2094
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