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6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7*
Compared to 5-DTAF, the 6-isomer is more preferably used for labeling oligonucleotides and nucleic acids. Like 5-DTAF, 6-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 6-DTAF is also used to label carbohydrates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 201.906 µL | 1.01 mL | 2.019 mL | 10.095 mL | 20.191 mL |
| 5 mM | 40.381 µL | 201.906 µL | 403.812 µL | 2.019 mL | 4.038 mL |
| 10 mM | 20.191 µL | 100.953 µL | 201.906 µL | 1.01 mL | 2.019 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9* | 493 | 517 | 83000 | 0.178 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
| 6-CR110 [6-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 6-CR6G [6-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5* | 552 | 578 | 90000 | 0.178 |
| 6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7* | 578 | 604 | 82000 | 0.168 |
| 6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* | 544 | 570 | 100000 | 0.34 |
References
View all 30 references: Citation Explorer
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Authors: Krahn KN, Bouten CV, van Tuijl S, van Z and voort MA, Merkx M.
Journal: Anal Biochem (2006): 177
Authors: Krahn KN, Bouten CV, van Tuijl S, van Z and voort MA, Merkx M.
Journal: Anal Biochem (2006): 177
Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129
Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481
Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285
Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O'Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253
Authors: Corbett MC, O'Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253
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