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7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester
7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is an amine-reactive blue fluorophore that has pH-dependent and environment-sensitive fluorescence. It is widely used for preparing bioconjugates of blue fluorescence.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 301.859 µL | 1.509 mL | 3.019 mL | 15.093 mL | 30.186 mL |
| 5 mM | 60.372 µL | 301.859 µL | 603.719 µL | 3.019 mL | 6.037 mL |
| 10 mM | 30.186 µL | 150.93 µL | 301.859 µL | 1.509 mL | 3.019 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 1 references: Citation Explorer
Sensitivity enhancement of fluorescent pH indicators using pH-dependent energy transfer
Authors: Gabor G, et al.
Journal: Anal Chim Acta (1995): 131
Authors: Gabor G, et al.
Journal: Anal Chim Acta (1995): 131
Page updated on November 21, 2024
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