5-TAMRA alkyne

5-TAMRA alkyne is an excellent azide-reactive fluorescent reagent for labeling alkyne-containing biological molecules through the well known click chemistry. It has the spectral properties identical to those of TRITC conjugates, thus all the instrument settings of the TRITC conjugates would be applicable to the applications of 5-TAMRA alkyne conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	213.895 µL	1.069 mL	2.139 mL	10.695 mL	21.389 mL
5 mM	42.779 µL	213.895 µL	427.789 µL	2.139 mL	4.278 mL
10 mM	21.389 µL	106.947 µL	213.895 µL	1.069 mL	2.139 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5-FAM Alkyne	493	517	83000	0.32	0.178
Cyanine 5 alkyne [equivalent to Cy5® alkyne]	651	670	250000¹	0.02	0.03
5-TAMRA ethylenediamine	552	578	90000	0.32	0.178
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-68-7	552	578	90000	0.32	0.178
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] CAS 154480-30-5	552	578	90000	0.32	0.178
6-TAMRA alkyne	552	578	90000	0.32	0.178

Citations

View all 4 citations: Citation Explorer

Suppression of ATG4B by copper inhibits autophagy and involves in Mallory body formation
Authors: Xia, Fan and Fu, Yuanyuan and Xie, Huazhong and Chen, Yuxin and Fang, Dongmei and Zhang, Wei and Liu, Peiqing and Li, Min
Journal: Redox biology (2022): 102284

Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471

Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer

Metal-enhanced fluorescence-based RNA sensing
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206

G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428

Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679

Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835

Page updated on November 21, 2024

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Catalog Number	487961
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Physical properties

Molecular weight	467.52
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501