5-Carboxyrhodamine 6G maleimide

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Physical properties

Molecular weight	765.74
Solvent	DMSO

Spectral properties

Absorbance (nm)	530
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	525
Emission (nm)	548
Quantum yield	0.95¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

765.74

Absorbance (nm)

530

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

525

Emission (nm)

548

Quantum yield

0.95¹

5-CR6G maleimide is a thiol-reactive derivative of rhodamine 6G. It is preferred choice (than the mixed isomers) for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. Rhodamine 6G is one of the brightest rhodamine dyes, but most of the rhodamine 6G dyes have much lower solubility than other rhodamine dyes. For similar or equivalent spectral properties, iFluor dyes have much better water solubilities.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxyrhodamine 6G maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	130.593 µL	652.963 µL	1.306 mL	6.53 mL	13.059 mL
5 mM	26.119 µL	130.593 µL	261.185 µL	1.306 mL	2.612 mL
10 mM	13.059 µL	65.296 µL	130.593 µL	652.963 µL	1.306 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)	530
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	525
Emission (nm)	548
Quantum yield	0.95¹

Images

Figure 1. Chemical structure for 5-Caroxyrhodamine 6G maleimide.

References

View all 50 references: Citation Explorer

Schwann Cell Migration through Magnetic Actuation Mediated by Fluorescent-Magnetic Bifunctional Fe3O4·Rhodamine 6G@Polydopamine Superparticles.
Authors: Wang, Yang and Du, Shulin and Liu, Ting and Ren, Jingyan and Zhang, Jiayi and Xu, Hao and Zhang, Hao and Liu, Yi and Lu, Laijin
Journal: ACS chemical neuroscience (2020): 1359-1370

Graphene-like BN@SiO2 nanocomposites as efficient sorbents for solid-phase extraction of Rhodamine B and Rhodamine 6G from food samples.
Authors: Chao, Yanhong and Pang, Jingyu and Bai, Yan and Wu, Peiwen and Luo, Jing and He, Jing and Jin, Yan and Li, Xiaowei and Xiong, Jun and Li, Huaming and Zhu, Wenshuai
Journal: Food chemistry (2020): 126666

Development of Rhodamine 6G-Based Fluorescent Chemosensors for Al3+-Ion Detection: Effect of Ring Strain and Substituent in Enhancing Its Sensing Performance.
Authors: Mandal, Jayanta and Ghorai, Pravat and Pal, Kunal and Bhaumik, Tanurima and Karmakar, Parimal and Saha, Amrita
Journal: ACS omega (2020): 145-157

A Fluorescent and Colorimetric Chemosensor for Hg2+ Based on Rhodamine 6G With a Two-Step Reaction Mechanism.
Authors: Bai, Cui-Bing and Wang, Wei-Gang and Zhang, Jie and Wang, Chang and Qiao, Rui and Wei, Biao and Zhang, Lin and Chen, Shui-Sheng and Yang, Song
Journal: Frontiers in chemistry (2020): 14

Growth and elongation of axons through mechanical tension mediated by fluorescent-magnetic bifunctional Fe3O4·Rhodamine 6G@PDA superparticles.
Authors: Wang, Yang and Li, Binxi and Xu, Hao and Du, Shulin and Liu, Ting and Ren, Jingyan and Zhang, Jiayi and Zhang, Hao and Liu, Yi and Lu, Laijin
Journal: Journal of nanobiotechnology (2020): 64

Application of Doehlert Matrix for an Optimized Preparation of a Surface-Enhanced Raman Spectroscopy (SERS) Substrate Based on Silicon Nanowires for Ultrasensitive Detection of Rhodamine 6G.
Authors: Ouhibi, Awatef and Saadaoui, Maroua and Lorrain, Nathalie and Guendouz, Mohammed and Raouafi, Noureddine and Moadhen, Adel
Journal: Applied spectroscopy (2020): 168-177

Removal of rhodamine 6G from synthetic effluents using Clitoria fairchildiana pods as low-cost biosorbent.
Authors: da Silva, Andreza Miranda Barata and Serrão, Naiany Oliveira and de Gusmão Celestino, Gustavo and Takeno, Mitsuo Lopes and Antunes, Nicanor Tiago Bueno and Iglauer, Stefan and Manzato, Lizandro and de Freitas, Flávio Augusto and Maia, Paulo José Sousa
Journal: Environmental science and pollution research international (2020): 2868-2880

Ultrasensitive SERS detection of rhodamine 6G and p-nitrophenol based on electrochemically roughened nano-Au film.
Authors: Wang, Jiangcai and Qiu, Cuicui and Mu, Xijiao and Pang, Hua and Chen, Xinchun and Liu, Dameng
Journal: Talanta (2020): 120631

Two-Photon-Induced Fluorescence Study of Rhodamine-6G Dye in Different Sets of Binary Solvents.
Authors: Gupta, Rahul Kumar and Kant, Surya and Rawat, Ashwini Kumar and Goswami, Debabrata
Journal: Journal of fluorescence (2020): 1043-1048

Photocatalytic Degradation of Aqueous Rhodamine 6G Using Supported TiO2 Catalysts. A Model for the Removal of Organic Contaminants From Aqueous Samples.
Authors: Pino, Eduardo and Calderón, Cristian and Herrera, Francisco and Cifuentes, Gerardo and Arteaga, Gisselle
Journal: Frontiers in chemistry (2020): 365