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5-(and-6)-Carboxy SNARF-5F Maleimide
5-(and-6)-Carboxy SNARF-5F Maleimide is an excellent thiol-reactive building block that can be readily used for developing SNARF-5F pH probes. It can be readily conjugated to peptides, antibodies and other thiol-containing biomolecules such as thiol-modified oligos. SNARF-5F has pKa of ∼7.2. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.
References
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Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe.
Authors: Nakata, Eiji and Gerelbaatar, Khongorzul and Komatsubara, Futa and Morii, Takashi
Journal: Molecules (Basel, Switzerland) (2022)
Authors: Nakata, Eiji and Gerelbaatar, Khongorzul and Komatsubara, Futa and Morii, Takashi
Journal: Molecules (Basel, Switzerland) (2022)
Durability of Implanted Low-Density Polyacrylamide Hydrogel Used as a Scaffold for Microencapsulated Molecular Probes inside Small Fish.
Authors: Shchapova, Ekaterina and Titov, Evgeniy and Gurkov, Anton and Nazarova, Anna and Borvinskaya, Ekaterina and Timofeyev, Maxim
Journal: Polymers (2022)
Authors: Shchapova, Ekaterina and Titov, Evgeniy and Gurkov, Anton and Nazarova, Anna and Borvinskaya, Ekaterina and Timofeyev, Maxim
Journal: Polymers (2022)
Evaluation of Interfacial pH Using Surface Forces Apparatus Fluorescence Spectroscopy.
Authors: Kasuya, Motohiro and Sano, Yuka and Kawashima, Masataka and Kurihara, Kazue
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 5073-5080
Authors: Kasuya, Motohiro and Sano, Yuka and Kawashima, Masataka and Kurihara, Kazue
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 5073-5080
A semi-naphthorhodafluor-based red-emitting fluorescent probe for tracking of hydrogen polysulfide in living cells and zebrafish.
Authors: Ma, Yingying and Xu, Zhencai and Sun, Qi and Wang, Linlin and Liu, Heng and Yu, Fabiao
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119105
Authors: Ma, Yingying and Xu, Zhencai and Sun, Qi and Wang, Linlin and Liu, Heng and Yu, Fabiao
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119105
Control of interfacial pH in mesoporous silica nanoparticles via surface functionalization.
Authors: Singappuli-Arachchige, Dilini and Slowing, Igor I
Journal: The Journal of chemical physics (2020): 034703
Authors: Singappuli-Arachchige, Dilini and Slowing, Igor I
Journal: The Journal of chemical physics (2020): 034703
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