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5-(and-6)-Carboxy SNARF-4F Succinimidyl Ester

5-(and-6)-Carboxy SNARF-4F Succinimidyl Ester is an excellent amine-reactive building block that can be readily used for developing SNARF-4F pH probes. SNARF-4F has pKa of ∼6.4. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.

References

View all 17 references: Citation Explorer
Biofilm-responsive encapsulated-phage coating for autonomous biofouling mitigation in water storage systems.
Authors: Zuo, Pengxiao and Metz, Jordin and Yu, Pingfeng and Alvarez, Pedro J J
Journal: Water research (2022): 119070
Non-Destructive Measurement of Acetic Acid and Its Distribution in a Photovoltaic Module during Damp Heat Testing Using pH-Sensitive Fluorescent Dye Sensors.
Authors: Nagasaki, Hideaki and Asaka, Takashi and Iwami, Kentaro and Umeda, Norihiro and Yamamoto, Chizuko and Hara, Yukiko and Masuda, Atsushi
Journal: Sensors (Basel, Switzerland) (2022)
Evaluation of Interfacial pH Using Surface Forces Apparatus Fluorescence Spectroscopy.
Authors: Kasuya, Motohiro and Sano, Yuka and Kawashima, Masataka and Kurihara, Kazue
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 5073-5080
Identifying the localization and exploring a functional role for Gprc5c in the kidney.
Authors: Rajkumar, Premraj and Cha, Boyoung and Yin, Jianyi and Arend, Lois J and Păunescu, Teodor G and Hirabayashi, Yoshio and Donowitz, Mark and Pluznick, Jennifer L
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2018): 2046-2059
Both NHERF3 and NHERF2 are necessary for multiple aspects of acute regulation of NHE3 by elevated Ca2+, cGMP, and lysophosphatidic acid.
Authors: Avula, Leela Rani and Chen, Tiane and Kovbasnjuk, Olga and Donowitz, Mark
Journal: American journal of physiology. Gastrointestinal and liver physiology (2018): G81-G90
Page updated on January 31, 2025

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Catalog Number21171
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Physical properties

Molecular weight

568.51

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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