ReadiCleave™ SSL biotin NHS ester

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	504.64
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

122266-55-1

Molecular weight

504.64

ReadiCleave™ SSL biotin NHS ester is a cleavable biotinylating reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. This biotin compound contains a cleavable linker that can be cleaved using reducing agents such as DTT or TCEP to remove the biotin label when needed.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ SSL biotin NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	198.161 µL	990.805 µL	1.982 mL	9.908 mL	19.816 mL
5 mM	39.632 µL	198.161 µL	396.322 µL	1.982 mL	3.963 mL
10 mM	19.816 µL	99.081 µL	198.161 µL	990.805 µL	1.982 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ReadiCleave™ Cy5-SSL-NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
ReadiCleave™ XFD532 SSL-NHS ester	534	553	81000	0.61¹	0.24	0.09
ReadiCleave™ FAM SSL-NHS ester	491	516	73000	0.92	-	0.35

Images

Figure 1. Chemical structure for ReadiCleave™ SSL biotin NHS ester.

References

View all 48 references: Citation Explorer

In Situ Membrane Biotinylation Enables the Direct Labeling and Accurate Kinetic Analysis of Small Extracellular Vesicles in Circulation.
Authors: Yu, Zi-Li and Zhao, Yi and Miao, Fan and Wu, Min and Xia, Hou-Fu and Chen, Zhuo-Kun and Liu, Hai-Ming and Zhao, Yi-Fang and Chen, Gang
Journal: Analytical chemistry (2021): 10862-10870

Off-the-shelf proximity biotinylation for interaction proteomics.
Authors: Santos-Barriopedro, Irene and van Mierlo, Guido and Vermeulen, Michiel
Journal: Nature communications (2021): 5015

Coupling of Cell Surface Biotinylation and SILAC-Based Quantitative Proteomics Identified Myoferlin as a Potential Therapeutic Target for Nasopharyngeal Carcinoma Metastasis.
Authors: Li, Maoyu and Peng, Fang and Wang, Guoqiang and Liang, Xujun and Shao, Meiying and Chen, Zhuchu and Chen, Yongheng
Journal: Frontiers in cell and developmental biology (2021): 621810

Proximity-Dependent Biotinylation (BioID) of Integrin Interaction Partners.
Authors: Myllymäki, Satu-Marja and Liu, Xiaonan and Varjosalo, Markku and Manninen, Aki
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 57-69

Proximity-Dependent Biotinylation to Elucidate the Interactome of TNK2 Nonreceptor Tyrosine Kinase.
Authors: Tahir, Raiha and Madugundu, Anil K and Udainiya, Savita and Cutler, Jevon A and Renuse, Santosh and Wang, Li and Pearson, Nicole A and Mitchell, Christopher J and Mahajan, Nupam and Pandey, Akhilesh and Wu, Xinyan
Journal: Journal of proteome research (2021): 4566-4577

Thiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics.
Authors: Li, Haorong and Frankenfield, Ashley M and Houston, Ryan and Sekine, Shiori and Hao, Ling
Journal: Journal of the American Society for Mass Spectrometry (2021): 2358-2365

Phenoxy Radical Reactivity of Nucleic Acids: Practical Implications for Biotinylation.
Authors: Wilbanks, Brandon and Garcia, Brian and Byrne, Shane and Dedon, Peter and Maher, L James
Journal: Chembiochem : a European journal of chemical biology (2021): 1400-1404

4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA.
Authors: Huang, Jinguo and Zhao, Ruiqi and Qin, Shanshan and Yang, Shixi and Li, Wei and Mo, Jing and Wang, Fang and Du, Yuhao and Weng, Xiaocheng and Zhou, Xiang
Journal: Chembiochem : a European journal of chemical biology (2021): 212-216

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells.
Authors: Zellner, Susanne and Nalbach, Karsten and Behrends, Christian
Journal: STAR protocols (2021): 100506

Rapid Enzyme-Mediated Biotinylation for Cell Surface Proteome Profiling.
Authors: Li, Yanan and Wang, Yan and Yao, Yating and Lyu, Jiawen and Qiao, Qinglong and Mao, Jiawei and Xu, Zhaochao and Ye, Mingliang
Journal: Analytical chemistry (2021): 4542-4551