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3-Maleimidopropionic acid N-hydroxysuccinimide ester *CAS 55750-62-4*
3-Maleimidopropionic acid N-hydroxysuccinimide ester is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive succinimidyl ester (SE) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The SE group of 3-maleimidopropionic acid N-hydroxysuccinimide ester is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. 3-Maleimidopropionic acid N-hydroxysuccinimide ester is a reagent that is widely used for generating stable maleimide-activated carrier proteins that can spontaneously react with sulfhydryls. Alternatively these relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 3-Maleimidopropionic acid N-hydroxysuccinimide ester *CAS 55750-62-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 375.643 µL | 1.878 mL | 3.756 mL | 18.782 mL | 37.564 mL |
| 5 mM | 75.129 µL | 375.643 µL | 751.287 µL | 3.756 mL | 7.513 mL |
| 10 mM | 37.564 µL | 187.822 µL | 375.643 µL | 1.878 mL | 3.756 mL |
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Citations
View all 1 citations: Citation Explorer
N-glycan targeted gene delivery to the dendritic cell SIGN receptor
Authors: Anderson, Kevin and Fern, undefined and ez, Christian and Rice, Kevin G
Journal: Bioconjugate chemistry (2010): 1479--1485
Authors: Anderson, Kevin and Fern, undefined and ez, Christian and Rice, Kevin G
Journal: Bioconjugate chemistry (2010): 1479--1485
References
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Journal: Photochem Photobiol Sci (2009): 1202
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Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
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Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
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Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
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Journal: Bioconjug Chem (2009): 702
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
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