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Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein*
Tide Fluor™ 2 (TF2) family has the spectral properties similar to those of fluoresceins and Alexa Fluor® 488 (Alexa Fluor® is the trademark of Invitrogen). Compared to fluoresceins TF2 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluoresceins. TF2-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with FITC or FAM. In pairing with our Tide Quencher™ 2 (TQ2), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. In addition, TF2 has spectral properties, pH dependence and photostability similar to Alexa Fluor® 488, making them excellent replacement for the expensive Alexa Fluor® 488.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 171.063 µL | 855.315 µL | 1.711 mL | 8.553 mL | 17.106 mL |
| 5 mM | 34.213 µL | 171.063 µL | 342.126 µL | 1.711 mL | 3.421 mL |
| 10 mM | 17.106 µL | 85.531 µL | 171.063 µL | 855.315 µL | 1.711 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| Tide Fluor™ 1 succinimidyl ester [TF1 SE]*Superior replacement for EDANS* | 341 | 447 | 20000 | 0.911 | 0.187 |
| Tide Fluor™ 3 succinimidyl ester [TF3 SE]*Superior replacement for Cy3* | 553 | 578 | 750001 | 0.11 | 0.179 |
| Tide Fluor™ 5WS succinimidyl ester [TF5WS SE]*Superior replacement for Cy5* | 649 | 663 | 250000 | 0.271 | 0.027 |
| Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red* | 577 | 602 | 90000 | 0.771 | 0.436 |
| Tide Fluor™ 6WS succinimidyl ester [TF6WS SE]*Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.271 | 0.101 |
| Tide Fluor™ 7WS, succinimidyl ester [TF7WS SE]*Superior replacement for Cy7* | 756 | 780 | 275000 | 0.31 | 0.049 |
| Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission* | 785 | 801 | 250000 | 0.211 | 0.109 |
| Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] *Superior replacement for Cy3* | 550 | 563 | 150000 | 0.151 | 0.079 |
| Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] *Superior replacement for FITC* | 491 | 516 | 75000 | 0.91 | 0.11 |
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Citations
View all 11 citations: Citation Explorer
Intraocular Delivery of a Collagen Mimetic Peptide Repairs Retinal Ganglion Cell Axons in Chronic and Acute Injury Models
Authors: Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J
Journal: International Journal of Molecular Sciences (2022): 2911
Authors: Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J
Journal: International Journal of Molecular Sciences (2022): 2911
Analysis of critical quality attributes in monoclonal antibodies for upstream process development
Authors: Oshinbolu, Sheun
Journal: (2019)
Authors: Oshinbolu, Sheun
Journal: (2019)
Cloning and characteristic of MMP1 gene from Hyriopsis cumingii and collagen hydrolytic activity of its recombinant protein
Authors: Hu, Baoqing and Xiao, Jun and Yi, Peipei and Hu, Chenxi and Zhu, Mingxing and Yin, Shuyuan and Wen, Chungen and Wu, Jielian
Journal: Gene (2019)
Authors: Hu, Baoqing and Xiao, Jun and Yi, Peipei and Hu, Chenxi and Zhu, Mingxing and Yin, Shuyuan and Wen, Chungen and Wu, Jielian
Journal: Gene (2019)
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
References
View all 25 references: Citation Explorer
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
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