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Summary of the components required for PCR
| PCR Component ▲ ▼ | Function ▲ ▼ |
| DNA Template | This is the sample DNA that contains the target sequence to be amplified. |
| Thermostable DNA polymerase | The enzyme that catalyzes the formation of new DNA strands complementary to the target sequence. Commonly used polymerases include TaqDNA polymerase and PfuDNA polymerase. |
| Primers (forward and reverse) | Short, single-stranded DNA sequences that hybridize to the sample DNA and start the process of replication. Primers are designed to complement the sequences at the beginning and end of the DNA template intended for amplifying. |
| Deoxynucleotide triphosphates (dNTPs) | These are the "building blocks" from which the DNA fragments will be synthesized and are commonly available in a ready-to-use format, such as ReadiUse™ dNTP Mix *10 mM* (Cat No. 17200) |
| Reaction buffer containing magnesium | This provides a stable environment for the PCR reaction. It has a suitable pH of 8.0 to 9.5 and is fortified with magnesium chloride, a co-factor of DNA polymerase. |
References
This online tool may be cited as follows
MLA | "Quest Database™ Summary of the components required for PCR." AAT Bioquest, Inc., 23 Apr. 2025, https://www.aatbio.com/data-sets/summary-of-the-components-required-for-pcr. | |
APA | AAT Bioquest, Inc. (2025, April 23). Quest Database™ Summary of the components required for PCR. AAT Bioquest. https://www.aatbio.com/data-sets/summary-of-the-components-required-for-pcr. | |
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