Fluorochrome Brightness Reference for Flow Cytometry

Name: Fluorochrome Brightness Reference for Flow Cytometry
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Brightness scale is 1 (dimmest) to 10 (brightest). Brightness is computed as a function of extinction coefficient and quantum yield.

A PDF version of this chart is available for download here.

Fluorophore ▲ ▼	Spectrum Viewer ▲ ▼	Ex ▲ ▼	Em ▲ ▼	Filter(nm) ▲ ▼	Laser Line ▲ ▼	Brightness ▲ ▼
Alexa Fluor 350	Link	346	442	450/40	355	2
iFluor 350	Link	345	450	450/40	355	6
mFluor UV375	Link	351	387	450/40	355	2
mFluor UV455	Link	357	461	450/40	355	2
mFluor UV460	Link	358	456	450/40	355	2
mFluor Violet 505	Link	393	504	450/40	405	6
mFluor Violet 545	Link	393	543	450/40	405	2
Pacific Orange	Link	400	551	525/50	405	2
mFluor Violet 540	Link	402	535	450/40	405	2
mFluor Violet 450	Link	406	445	450/50	405	5
Pacific Blue	Link	410	455	450/40	405	2
mFluor Violet 500	Link	410	501	510/80	445	6
mFluor Violet 510	Link	412	505	525/50	405	5
mFluor Violet 610	Link	421	613	450/50	405	3
iFluor 450	Link	451	502	530/30	405	5
Pacific Green	Link	410	500	530/30	405	5
iFluor 405	Link	403	427	450/40	405	6
iFluor 430	Link	433	498	530/30	405	6
mFluor Violet 550	Link	527	550	525/50	532	5
mFluor UV540	Link	542	560	525/50	532	6
mFluor Violet 590	Link	564	591	525/50	561	5
Alexa Fluor 555	Link	555	580	525/50	488	4
Cy3	Link	554	568	525/50	488	4
Alexa Fluor 488	Link	490	525	530/30	488	5
FITC	Link	490	525	530/30	488	7
iFluor 488	Link	491	516	530/30	488	7
Oregon Green	Link	501	526	530/30	488	7
TRITC	Link	557	576	525/50	488	7
PE	Link	565	578	575/26	488	10
mFluor Blue 590	Link	569	589	575/26	561	5
Alexa Fluor 555	Link	555	580	525/50	561	5
Cy3	Link	554	568	525/50	561	5
mFluor Blue 620	Link	589	616	525/50	561	5
mFluor UV610	Link	590	609	610/20	561	7
Alexa Fluor 594	Link	590	617	610/20	561	6
iFluor 610	Link	610	628	610/20	561	6
Texas Red	Link	596	615	610/20	561	6
Alexa Fluor 532	Link	532	554	525/50	561	7
iFluor 514	Link	528	555	525/50	561	7
iFluor 532	Link	544	564	525/50	561	7
iFluor 546	Link	541	557	525/50	561	7
iFluor 555	Link	557	570	525/50	561	7
iFluor 568	Link	568	587	525/50	561	7
iFluor 594	Link	588	604	610/20	561	7
iFluor 790	Link	787	812	780/60	640	3
iFluor 800	Link	801	820	Custom	640	3
iFluor 810	Link	811	822	Custom	640	3
iFluor 820	Link	822	850	Custom	640	3
iFluor 860	Link	853	878	Custom	640	3
Alexa Fluor 750	Link	749	775	780/60	640	4
iFluor 750	Link	757	779	780/60	640	4
iFluor 680	Link	684	701	Custom	640	5
Alexa Fluor 680	Link	679	702	695/40	640	6
Alexa Fluor 700	Link	702	723	720/30	640	6
Cy7	Link	756	779	780/60	640	6
iFluor 647	Link	656	670	660/20	640	6
iFluor 660	Link	663	678	660/20	640	6
iFluor 700	Link	690	713	Custom	640	6
Alexa Fluor 647	Link	650	665	660/20	640	7
Cy5	Link	649	666	660/20	640	7
iFluor 633	Link	640	654	660/20	640	7
APC	Link	650	660	660/20	640	9
PE-Alexa Fluor 647	Link	568	666	660/20	488	7
PE-Cy5.5	Link	566	671	695/40	488	7
PE-Alexa Fluor 594	Link	566	618	610/20	488	9
PE-Alexa Fluor 750	Link	566	775	780/60	488	9
PE-iFluor 594	Link	566	604	610/20	488	9
PE-iFluor 750	Link	566	778	780/60	488	9
PE-Cy5	Link	565	666	660/20	488	10
PE-Cy7	Link	566	778	780/60	488	10
PE-iFluor 647	Link	569	666	660/20	488	10
PE-Texas Red	Link	567	615	610/20	488	10
APC-Cy7	Link	651	779	780/60	633	5
APC-Alexa Fluor 750	Link	650	775	780/60	633	8
APC-Cy5.5	Link	656	700	695/40	633	8
APC-iFluor 700	Link	651	710	780/60	633	8
APC-iFluor 750	Link	651	791	780/60	633	8

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References

This online tool may be cited as follows

MLA	"Quest Database™ Fluorochrome Brightness Reference for Flow Cytometry." AAT Bioquest, Inc., 23 Nov. 2024, https://www.aatbio.com/data-sets/fluorochrome-brightness-reference-for-flow-cytometry.
APA	AAT Bioquest, Inc. (2024, November 23). Quest Database™ Fluorochrome Brightness Reference for Flow Cytometry. AAT Bioquest. https://www.aatbio.com/data-sets/fluorochrome-brightness-reference-for-flow-cytometry.
BibTeX	EndNote	RefMan

Fluorochrome Brightness Reference for Flow Cytometry

References

This online tool may be cited as follows

MLA

APA