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Analysis of bovine serum albumin unfolding in the absence and presence of ATP by SYPRO Orange staining of agarose native gel electrophoresis
Authors: Tomioka, Yui and Nakagawa, Masataka and Sakuma, Chiaki and Kurosawa, Yasunori and Nagatoishi, Satoru and Tsumoto, Kouhei and Arakawa, Tsutomu and Akuta, Teruo
Journal: Analytical Biochemistry (2022): 114817

Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy.
Authors: Kirley, Terence L and Norman, Andrew B
Journal: Biochemical and biophysical research communications (2021): 93-98

Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA.
Authors: Leitner, Peter D and Vietor, Ilja and Huber, Lukas A and Valovka, Taras
Journal: Scientific reports (2021): 2331

Thermal Shift Assay for Exploring Interactions Between Fatty Acid-Binding Protein and Inhibitors.
Authors: Hao, Jiaqing
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 395-409

Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.
Authors: Crossen, J and Diamond, S L
Journal: Biochimica et biophysica acta. General subjects (2021): 129805

SYPRO Orange - a new gold standard amyloid probe.
Authors: Mora, Aruna K and Nath, Sukhendu
Journal: Journal of materials chemistry. B (2020): 7894-7898

A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.
Authors: Kirley, Terence L and Norman, Andrew B and Wetzel, Hanna N
Journal: Journal of immunological methods (2020): 112676

nanoDSF: In vitro Label-Free Method to Monitor Picornavirus Uncoating and Test Compounds Affecting Particle Stability.
Authors: Real-Hohn, Antonio and Groznica, Martin and Löffler, Nadine and Blaas, Dieter and Kowalski, Heinrich
Journal: Frontiers in microbiology (2020): 1442

Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes.
Authors: Rieser, Ruth and Penaud-Budloo, Magalie and Bouzelha, Mohammed and Rossi, Axel and Menzen, Tim and Biel, Martin and Büning, Hildegard and Ayuso, Eduard and Winter, Gerhard and Michalakis, Stylianos
Journal: Journal of pharmaceutical sciences (2020): 854-862

Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants.
Authors: Oshinbolu, Sheun and Shah, Rachana and Finka, Gary and Molloy, Mike and Uden, Mark and Bracewell, Daniel G
Journal: Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) (2018): 909-917

Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487.
Authors: Fiebig, David and Storka, Juliana and Roeder, Markus and Meyners, Christian and Schmelz, Stefan and Blankenfeldt, Wulf and Scrima, Andrea and Kolmar, Harald and Fuchsbauer, Hans-Lothar
Journal: The FEBS journal (2018): 4246-4264

Differential scanning calorimetry and fluorimetry measurements of monoclonal antibodies and reference proteins: Effect of scanning rate and dye selection.
Authors: Lang, Brian E and Cole, Kenneth D
Journal: Biotechnology progress (2017): 677-686

Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay.
Authors: Lucet, Isabelle S and Murphy, James M
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 91-104

EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay.
Authors: Kroeger, Tobias and Frieg, Benedikt and Zhang, Tao and Hansen, Finn K and Marmann, Andreas and Proksch, Peter and Nagel-Steger, Luitgard and Groth, Georg and Smits, Sander H J and Gohlke, Holger
Journal: PloS one (2017): e0177024

Strategies for over-expression and purification of recombinant full length STAT5B in Escherichia coli.
Authors: de Araujo, Elvin D and Geletu, Mulu and Gunning, Patrick T
Journal: Protein expression and purification (2017): 1-8

High-throughput thermofluor-based assays for inhibitor screening of STAT SH2 domains.
Authors: de Araujo, Elvin D and Manaswiyoungkul, Pimyupa and Israelian, Johan and Park, Jisung and Yuen, Karen and Farhangi, Shiva and Berger-Becvar, Angelika and Abu-Jazar, Lubna and Gunning, Patrick T
Journal: Journal of pharmaceutical and biomedical analysis (2017): 159-167

Pyrrolobenzodiazepine Antibody-Drug Conjugates Designed for Stable Thiol Conjugation.
Authors: Christie, R James and Tiberghien, Arnaud C and Du, Qun and Bezabeh, Binyam and Fleming, Ryan and Shannon, Amanda and Mao, Shenlan and Breen, Shannon and Zhang, Jing and Zhong, Haihong and Harper, Jay and Wu, Herren and Howard, Philip W and Gao, Changshou
Journal: Antibodies (Basel, Switzerland) (2017)

Binding mode of metal ions to the bacterial iron import protein EfeO.
Authors: Temtrirath, Kanate and Okumura, Kenji and Maruyama, Yukie and Mikami, Bunzo and Murata, Kousaku and Hashimoto, Wataru
Journal: Biochemical and biophysical research communications (2017): 1095-1101

Large-scale production of recombinant Saw1 in Escherichia coli.
Authors: Rashev, Margarita and Surtees, Jennifer A and Guarné, Alba
Journal: Protein expression and purification (2017): 75-80

Femtomole-Scale High-Throughput Screening of Protein Ligands with Droplet-Based Thermal Shift Assay.
Authors: Liu, Wen-Wen and Zhu, Ying and Fang, Qun
Journal: Analytical chemistry (2017): 6678-6685

Using thermal scanning assays to test protein-protein interactions of inner-ear cadherins.
Authors: Choudhary, Deepanshu and Kumar, Anusha and Magliery, Thomas J and Sotomayor, Marcos
Journal: PloS one (2017): e0189546

Characterization of aminoacyl-tRNA synthetase stability and substrate interaction by differential scanning fluorimetry.
Authors: Abbott, Jamie A and Livingston, Nathan M and Egri, Shawn B and Guth, Ethan and Francklyn, Christopher S
Journal: Methods (San Diego, Calif.) (2017): 64-71

An Ancestral Tryptophanyl-tRNA Synthetase Precursor Achieves High Catalytic Rate Enhancement without Ordered Ground-State Tertiary Structures.
Authors: Sapienza, Paul J and Li, Li and Williams, Tishan and Lee, Andrew L and Carter, Charles W
Journal: ACS chemical biology (2016): 1661-8

The dye SYPRO orange binds to amylin amyloid fibrils but not pre-fibrillar intermediates.
Authors: Wong, Amy G and Raleigh, Daniel P
Journal: Protein science : a publication of the Protein Society (2016): 1834-40

The Effect of Heat on the Physicochemical Properties of Bacteriophage MS2.
Authors: Brié, Adrien and Bertrand, Isabelle and Meo, Marie and Boudaud, Nicolas and Gantzer, Christophe
Journal: Food and environmental virology (2016): 251-261

Insights in understanding aggregate formation and dissociation in cation exchange chromatography for a structurally unstable Fc-fusion protein.
Authors: Chen, Zhiqiang and Huang, Chao and Chennamsetty, Naresh and Xu, Xuankuo and Li, Zheng Jian
Journal: Journal of chromatography. A (2016): 110-22

High-throughput screening of formulations to optimize the thermal stability of a therapeutic monoclonal antibody.
Authors: Niedziela-Majka, Anita and Kan, Elaine and Weissburg, Perry and Mehra, Upasana and Sellers, Scott and Sakowicz, Roman
Journal: Journal of biomolecular screening (2015): 552-9

Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase.
Authors: Silva, Walciane and Terra, Walter R and Ferreira, Clélia
Journal: Biochemistry and biophysics reports (2015): 215-223

Development of a Thermofluor assay for stability determination of membrane proteins using the Na(+)/H(+) antiporter NhaA and cytochrome c oxidase.
Authors: Kohlstaedt, Martin and von der Hocht, Iris and Hilbers, Florian and Thielmann, Yvonne and Michel, Hartmut
Journal: Acta crystallographica. Section D, Biological crystallography (2015): 1112-22

Analysis of protein stability and ligand interactions by thermal shift assay.
Authors: Huynh, Kathy and Partch, Carrie L
Journal: Current protocols in protein science (2015): 28.9.1-28.9.14

Identification of Bacillus anthracis PurE inhibitors with antimicrobial activity.
Authors: Kim, Anna and Wolf, Nina M and Zhu, Tian and Johnson, Michael E and Deng, Jiangping and Cook, James L and Fung, Leslie W-M
Journal: Bioorganic & medicinal chemistry (2015): 1492-9

A method for evaluating nucleosome stability with a protein-binding fluorescent dye.
Authors: Taguchi, Hiroyuki and Horikoshi, Naoki and Arimura, Yasuhiro and Kurumizaka, Hitoshi
Journal: Methods (San Diego, Calif.) (2014): 119-26

Ligand induced stabilization of the melting temperature of the HSV-1 single-strand DNA binding protein using the thermal shift assay.
Authors: Rupesh, Kanchi Ravi and Smith, Aaron and Boehmer, Paul E
Journal: Biochemical and biophysical research communications (2014): 604-8

Direct and simple detection of recombinant proteins from cell lysates using differential scanning fluorimetry.
Authors: Seo, Dong-Ho and Jung, Jong-Hyun and Kim, Hae-Yeong and Park, Cheon-Seok
Journal: Analytical biochemistry (2014): 75-80

Effects of subclass change on the structural stability of chimeric, humanized, and human antibodies under thermal stress.
Authors: Ito, Takahiko and Tsumoto, Kouhei
Journal: Protein science : a publication of the Protein Society (2013): 1542-51

Orthogonal high-throughput thermal scanning method for rank ordering protein formulations.
Authors: Nashine, Vishal C and Kroetsch, Andrew M and Sahin, Erinc and Zhou, Rong and Adams, Monica L
Journal: AAPS PharmSciTech (2013): 1360-6

Differential scanning fluorescence approach using a fluorescent molecular rotor to detect thermostability of proteins in surfactant-containing formulations.
Authors: Ablinger, Elisabeth and Leitgeb, Stefan and Zimmer, Andreas
Journal: International journal of pharmaceutics (2013): 255-60

Dilution of protein-surfactant complexes: a fluorescence study.
Authors: Azadi, Glareh and Chauhan, Anuj and Tripathi, Anubhav
Journal: Protein science : a publication of the Protein Society (2013): 1258-65

Heat stability of Proteobacterial PII protein facilitate purification using a single chromatography step.
Authors: Moure, Vivian R and Razzera, Guilherme and Araújo, Luíza M and Oliveira, Marco A S and Gerhardt, Edileusa C M and Müller-Santos, Marcelo and Almeida, Fabio and Pedrosa, Fabio O and Valente, Ana P and Souza, Emanuel M and Huergo, Luciano F
Journal: Protein expression and purification (2012): 83-88

Use of differential scanning fluorimetry as a high-throughput assay to identify nuclear receptor ligands.
Authors: DeSantis, Kara and Reed, Aaron and Rahhal, Raneen and Reinking, Jeff
Journal: Nuclear receptor signaling (2012): e002

High-throughput measurement, correlation analysis, and machine-learning predictions for pH and thermal stabilities of Pfizer-generated antibodies.
Authors: King, Amy C and Woods, Matthew and Liu, Wei and Lu, Zhijian and Gill, Davinder and Krebs, Mark R H
Journal: Protein science : a publication of the Protein Society (2011): 1546-57

Quantitation of protein-protein interactions by thermal stability shift analysis.
Authors: Layton, Curtis J and Hellinga, Homme W
Journal: Protein science : a publication of the Protein Society (2011): 1439-50

Formulation development of therapeutic monoclonal antibodies using high-throughput fluorescence and static light scattering techniques: role of conformational and colloidal stability.
Authors: Goldberg, Deborah S and Bishop, Steven M and Shah, Ambarish U and Sathish, Hasige A
Journal: Journal of pharmaceutical sciences (2011): 1306-15

Development of a differential scanning fluorimetry based high throughput screening assay for the discovery of affinity binders against an anthrax protein.
Authors: Sorrell, Fiona J and Greenwood, Gemma K and Birchall, Kristian and Chen, Beining
Journal: Journal of pharmaceutical and biomedical analysis (2010): 802-8

Detection of IgG aggregation by a high throughput method based on extrinsic fluorescence.
Authors: He, Feng and Phan, Duke H and Hogan, Sabine and Bailey, Robert and Becker, Gerald W and Narhi, Linda O and Razinkov, Vladimir I
Journal: Journal of pharmaceutical sciences (2010): 2598-608

SYPRO Orange fluorescent staining of protein gels.
Authors: Simpson, Richard J
Journal: Cold Spring Harbor protocols (2010): pdb.prot5414

Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes.
Authors: Layton, Curtis J and Hellinga, Homme W
Journal: Biochemistry (2010): 10831-41

Differential scanning fluorimetry measurement of protein stability changes upon binding to glycosaminoglycans: a screening test for binding specificity.
Authors: Uniewicz, Katarzyna A and Ori, Alessandro and Xu, Ruoyan and Ahmed, Yassir and Wilkinson, Mark C and Fernig, David G and Yates, Edwin A
Journal: Analytical chemistry (2010): 3796-802

Fabrication and fluorescence imaging of human low-density lipoprotein coatings for highly efficient capillary electrophoresis separation of basic proteins.
Authors: Yin, Junfa and Song, Yuling and Wang, Zhixin and Wang, Hailin
Journal: Electrophoresis (2009): 1362-71

Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area.
Authors: Krintel, Christian and Mörgelin, Matthias and Logan, Derek T and Holm, Cecilia
Journal: The FEBS journal (2009): 4752-62

Staining proteins in gels.
Authors: Gallagher, Sean and Chakavarti, Deb
Journal: Journal of visualized experiments : JoVE (2008)