Application Notes

LysoTracker dyes, which are fluorescent probes with weakly basic properties, readily accumulate within acidic organelles-particularly lysosomes and late endosomes-making them valuable for live cell imaging of these intracellular compartments. Lysosomes: Dynamic Hubs of Cellular Homeostasis…

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Posted January 6, 2025

RiboGreen assays, based on the RiboGreen dye's fluorescence-enhancement principle upon binding to RNA, provide a fluorometric method for RNA quantification in molecular biology and biopharmaceutical applications. Background In high-precision molecular biology and biopharmaceutical workflows,…

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Posted January 6, 2025

Black Hole Quenchers (BHQs) represent a sophisticated class of dark quencher molecules engineered for nucleic acid detection. Background Quenchers are chemical species used to suppress or prevent the fluorescence of a fluorophore by providing a pathway for energy or electron transfer away from…

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Posted December 17, 2024

GelRed is a nucleic acid intercalating fluorescent stain designed to prevent cell membrane penetration while maintaining high DNA binding affinity. Background and Development Developed by Biotium Inc., GelRed (Cat. No. 41003) represents an advancement in nucleic acid staining technology. The…

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Posted December 13, 2024

PicoGreen is a highly sensitive fluorescent probe developed by Invitrogen, specifically designed to detect double-stranded DNA (dsDNA, Dragan, A.I., et al., 2010). Upon binding to dsDNA, PicoGreen undergoes a conformational change that results in the formation of a fluorescent complex, emitting…

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Posted December 11, 2024