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Z-IETD-ProRed™ 620
ProRed™-derived protease substrates are colorless and non-fluorescent. Cleavage of blocking protease-cleavable peptide residue by caspases generates the strongly red fluorescent ProRed™ that can be monitored fluorimetrically at ~620 nm with excitation of ~530 nm. ProRed™-derived caspase substrates are the most sensitive red indicators for the fluorimetric detection of various caspase activities. This IETD-ProRed™ substrate is specific for detecting caspase 8.
Detection of Caspase 8 Activity in Jurkat cells with Z-IETD-ProRed™ 620. Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with staurosporine at the final concentration of 1 µM for 5 hours while the untreated cells were used as control. The caspase 8 assay solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm with a FlexStation™ microplate reader (Molecular Devices).
Detection of Caspase 8 Activity in Jurkat cells with Z-IETD-ProRed™ 620. Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with staurosporine at the final concentration of 1 µM for 5 hours while the untreated cells were used as control. The caspase 8 assay solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm with a FlexStation™ microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
134341 mg
Price
 
Physical properties
Molecular weight1565.59
SolventDMSO
Spectral properties
Excitation (nm)532
Emission (nm)619
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 26, 2025