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xtraFluor™ Violet 420 Streptavidin Conjugate

Product key features

  • Optimized to be maximally excited at 405 nm (Violet laser)
  • Optimized for multicolor flow cytometry applications
  • Spectrally compatible with the filter set of BV421
  • Probably the brightest violet laser-excitable streptavidin conjugate

Product description

xtraFluor™ Violet 420 Streptavidin Conjugate might be one of the brightest fluorescent dye-labeled streptavidin conjugates commercially available. It can be conveniently used for detecting biotin-tagged biomolecules such as biotinylated antibodies, peptides and oligos. XV420 is an extremely bright violet laser-excitable fluorophore. With a maximum excitation well matched the 405 nm violet laser and an emission peak at ~420 nm, XV420 can be conveniently used to replace BD Horizon Brilliant™ Violet 421 (BV421). It can be used on flow cytometers equipped with a violet laser and appropriate filters for large multicolor flow cytometry panels. It is a brighter alternative to Pacific Blue (ThermoFisher) and BD Horizon™ V450 (BD Biosciences). XV420 enables improved resolution for dim population identification. It is particularly useful in multicolor applications where it can be used to better resolve dim populations. XV420 dye offers new options for multicolor panel design through the use of a violet laser. Additionally, the dye’s spillover properties, stability under light and in standard buffers, and compatibility with blood collection tubes offer ease-of-use for a range of applications.

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
Biotin-xtraFluor™ Violet 420 Conjugate4074243,000,0000.067

References

View all 49 references: Citation Explorer
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy.
Authors: Sahi, Maryam and Andersson, Sarah and Mattson, Cecilia and Dale, Matilda and Kagiolglou, Sofia and Hofström, Camilla and Persson, Helena and Klingström, Jonas and Chiodi, Francesca and Fredolini, Claudia
Journal: Journal of visualized experiments : JoVE (2024)
Effects of solid retention time and exposure mode to electric current on Remazol Brilliant Violet removal in an electro-membrane bioreactor.
Authors: Belli, Tiago José and Bassin, João Paulo and de Sousa Vidal, Carlos Magno and Hassemer, Maria Eliza Nagel and Rodrigues, Caroline and Lapolli, Flávio Rubens
Journal: Environmental science and pollution research international (2023): 58412-58427
Sonophotocatalytic Degradation of Fast Yellow AB and Remazol Brilliant Violet-5R by Using Ag-Impregnated ZnO as a Photocatalyst.
Authors: Aziz, Nosheen and Rasool, Shagufta and Ullah, Toheed and Khitab, Fatima and Halim, Sobia Ahsan and Bawazeer, Sami and Khan, Ajmal and Al-Harrasi, Ahmed
Journal: ACS omega (2023): 18509-18515
Performance of treatment schemes comprising chromium-hydrogen peroxide-based advanced oxidation process for textile wastewater.
Authors: Mondal, Prasenjit and Mukherji, Suparna and Garg, Anurag
Journal: Environmental science and pollution research international (2022): 88089-88100
Comparative study and dyeing performance of as-synthesized azo heterocyclic monomeric, polymeric, and commercial disperse dyes.
Authors: Abdullahi, Shehu Sa'ad and Musa, Haruna and Habibu, Shehu and Birniwa, Abdullahi Haruna and Mohammad, Rania Edrees Adam
Journal: Turkish journal of chemistry (2022): 1841-1852
Page updated on April 25, 2025

Ordering information

Price
Unit size
Catalog Number70805
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
Top)The spectral profile was acquired using a 4-laser spectral flow cytometer equipped with spatially offset excitation sources at 355 nm, 405 nm, 488 nm, and 640 nm. Each laser produced a distinct emission pattern, and the combined emission spectra were used to generate the composite spectral signature. Bottom) Flow cytometric analysis of human whole blood cells stained with biotinylated anti-CD4 antibody (0.5 µg/test), followed by detection with xtraFluor™ Violet 420-streptavidin (0.25 µg/test). Fluorescence was measured using the V1-A detector channel on the Aurora spectral cytometer.
Top)The spectral profile was acquired using a 4-laser spectral flow cytometer equipped with spatially offset excitation sources at 355 nm, 405 nm, 488 nm, and 640 nm. Each laser produced a distinct emission pattern, and the combined emission spectra were used to generate the composite spectral signature. Bottom) Flow cytometric analysis of human whole blood cells stained with biotinylated anti-CD4 antibody (0.5 µg/test), followed by detection with xtraFluor™ Violet 420-streptavidin (0.25 µg/test). Fluorescence was measured using the V1-A detector channel on the Aurora spectral cytometer.
Top)The spectral profile was acquired using a 4-laser spectral flow cytometer equipped with spatially offset excitation sources at 355 nm, 405 nm, 488 nm, and 640 nm. Each laser produced a distinct emission pattern, and the combined emission spectra were used to generate the composite spectral signature. Bottom) Flow cytometric analysis of human whole blood cells stained with biotinylated anti-CD4 antibody (0.5 µg/test), followed by detection with xtraFluor™ Violet 420-streptavidin (0.25 µg/test). Fluorescence was measured using the V1-A detector channel on the Aurora spectral cytometer.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
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