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VIC phosphoramidite
VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3 phosphoramidite | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy5 phosphoramidite | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
References
View all 5 references: Citation Explorer
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chain reaction.
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4).
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Detection and quantitation of akabane and aino viruses by multiplex real-time reverse-transcriptase PCR.
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
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