Transfectamine™ mRNA Transfection Reagent
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Example protocol
AT A GLANCE
Prepare cells for transfection.
Prepare the Transfectamine™ mRNA Transfection Reagent-RNA mixture.
Add the Transfectamine™ mRNA Transfection Reagent-RNA mixture to the cell culture.
Culture cells overnight.
Analyze transfection efficiency with an appropriate method.
CELL PREPARATION
Culture cells to ~ 90% confluency at the time of transfection.
Replace with fresh growth medium before transfection. For example, replace with 2 mL of medium per well for 6-well plates and 6 mL of medium for 10 cm plates.
PREPARATION OF WORKING SOLUTION
Mix 2.5 µg of mRNA with 200 µL of serum-free medium.
Add 7.5 µL of Transfectamine™ mRNA Transfection Reagent to Step 1.
Mix well and incubate at RT for 20 minutes.
Note: The ratio of Transfectamine™ mRNA Transfection Reagent to mRNA needs to be optimized for different cell lines. In general, Transfectamine™ mRNA Transfection Reagent (µL) to mRNA (µg) Ratio = (3 to 5 µL) to 1 µg.
Table 1. Sample protocol detail for 6-well plates as shown in the table below.
Component | 6-well plate (per well) |
Fresh culture medium | 2 mL |
Purified mRNA | ~2.5 µg |
Serum-free medium | 200 µL |
Transfectamine™ mRNA Transfection Reagent | ~7.5 µL |
SAMPLE EXPERIMENTAL PROTOCOL
Add the Transfectamine™ mRNA Transfection Reagent -mRNA mixture to the culture plate and culture overnight.
Note: Recombinant protein expression can be detected as early as 8 hours after the transfection. Maximal expression level may be observed ~24 hours after the transfection.
Images
![Transfection efficiency comparison (Upper panel) and cellular toxicity comparison (Bottom panel) in HeLa cells. HeLa cells were cultured in a 6-well plate to ~90% confluency. 2.5 µg of mRNA was transfected with Lipofectamine MessengerMAX and Transfectamine™ mRNA Transfection Reagent, respectively. Images were taken 18 hours after the transfection using a fluorescent microscope with the FITC channel (Upper panel). Although transfection efficiency was similar for Lipofectamine MessengerMAX and Transfectamine™ mRNA Transfection Reagent, most Lipofectamine MessengerMAX transfected samples were scrambled, whereas cells transfected with Transfectamine™ mRNA Transfection Reagent looked much healthier (bottom panel).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftransfectamine-mrna-transfection-reagent%2Ffigure-for-transfectamine-mrna-transfection-reagent_vy6Pf.jpg&w=3840&q=75)
![Cell viability comparison in HeLa cells. HeLa cells were incubated with Transfectamine™ mRNA Transfection Reagent and Lipofectamine MessagerMax, respectively, according to suggested protocol without mRNA. After 24 hours, cell viability in both groups was measured with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit (Cat#22770). The higher absorbance at 460 represents more numbers of viable cells.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftransfectamine-mrna-transfection-reagent%2Ffigure-for-transfectamine-mrna-transfection-reagent_B30Ya.jpg&w=3840&q=75)
References
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