TAQuest™ qPCR Master Mix with Helixyte™ Green No ROX

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

Overview SDS Protocol

TAQuest™ qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix includes our proprietary TAQuest™ Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer. You only need to add template and target primers to run the desired PCR reactions. The Hot Start Taq DNA polymerase allows you to set up a PCR reaction at room temperature, thus minimizing non-specific product formation. In combination with an optimized buffer, the enzyme ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix does not contain a ROX reference dye.

Platform

qPCR

Instrument specification(s)

SYBR Green filter

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.
Note Thaw the TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* at room temperature. Vortex qPCR Master Mix thoroughly before use.

Prepare one of the following reaction mixes as indicated in Table 1.
Carefully mix the reagents with a gentle vortex followed by a brief centrifuge.
Set up the plate in the qPCR instrument and run as indicated in Table 2.

Table 1.Reagents composition per well for each reaction

Components	Volume (25 µL/reaction)	Volume (50 µL/reaction)	Final Conc.
TAQuest™ qPCR Master Mix with Helixyte™ Green No ROX	12.5 µL	25 µL	1X
Upstream primer, 10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
Downstream primer, 10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
DNA template	1-5 µL	1-5 µL	Optimized conc.
Nuclease-Free Water to	25 µL	50 µL

Table 2.Thermal cycling parameters

Parameter	Polymerase Activation	PCR (30-40 cycles)
	Hold	Denature	Anneal	Extend
Temperature	95 °C	95 °C	55-65 °C	68-72 °C
Time (m:ss)	0:20	0:30	1:00	1:00

Images

Figure 1. Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*.

References

View all 50 references: Citation Explorer

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Simultaneous detection and differentiation of porcine circovirus 3 and 4 using a SYBR Green І-based duplex quantitative PCR assay.
Authors: Hou, Cheng-Yao and Xu, Tong and Zhang, Liu-Hui and Cui, Jian-Tao and Zhang, Yuan-Hang and Li, Xin-Sheng and Zheng, Lan-Lan and Chen, Hong-Ying
Journal: Journal of virological methods (2021): 114152

Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus.
Authors: Kokane, Amol D and Lawrence, Kapil and Kokane, Sunil B and Gubyad, Mrugendra G and Misra, Pragati and Reddy, M Krishna and Ghosh, Dilip Kumar
Journal: 3 Biotech (2021): 359

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of a SYBR Green-based real-time quantitative polymerase chain reaction assay to detect enzootic nasal tumor virus in goats.
Authors: He, Rongze and Du, Yulan and Gan, Linli and Mohsin, Muhammad Ali and He, Bao-Xiang
Journal: Canadian journal of veterinary research = Revue canadienne de recherche veterinaire (2021): 145-150

Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7.
Authors: Li, Y D and Yu, Z D and Bai, C X and Zhang, D and Sun, P and Peng, M L and Liu, H and Wang, J and Wang, Y
Journal: Polish journal of veterinary sciences (2021): 43-49

SYBR Green real-time qPCR method: Diagnose drowning more rapidly and accurately.
Authors: Yu, Zhonghao and Xu, Quyi and Xiao, Cheng and Li, Huan and Wu, Weibin and Du, Weian and Zhao, Jian and Liu, Hong and Wang, Huijun and Liu, Chao
Journal: Forensic science international (2021): 110720