SO Green™ 520WS Singlet Oxygen Sensor *Water-Soluble for Extracellular Applications*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 910.52 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 910.52 |
SO Green™ 520WS Singlet Oxygen Sensor has been developed as a water-soluble fluorescent probe for detecting extracellular singlet oxygen since it is cell-impermeant. It is highly selective for singlet oxygen. Unlike other available fluorescent and chemiluminescent singlet oxygen detection reagents, SO Green™ 520WS Singlet Oxygen Sensor does not show any appreciable response to hydroxyl radical, superoxide, or other reactive oxygen species (ROS). This indicator only exhibits weak blue fluorescence. It emits a strong green fluorescence (excitation/emission maxima ∼504/525 nm) upon reaction with singlet oxygen. The PET quencher of SO Green™ 520WS Singlet Oxygen Sensor is eliminated (by singlet oxygen reaction) to recover its fluorescence. Singlet oxygen can be produced from many different sources such as dye photosensitizations. In mammalian biology, singlet oxygen is one of the ROS, which is linked to oxidation of LDL cholesterol and resultant cardiovascular effects. Polyphenol antioxidants can scavenge and reduce concentrations of reactive oxygen species and may prevent such deleterious oxidative effects. Ingestion of pigments capable of producing singlet oxygen with activation by light can produce severe photosensitivity of skin. This is especially a concern in herbivorous animals. Singlet oxygen is considered to be the active species in photodynamic therapy.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of SO Green™ 520WS Singlet Oxygen Sensor *Water-Soluble for Extracellular Applications* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 109.827 µL | 549.137 µL | 1.098 mL | 5.491 mL | 10.983 mL |
5 mM | 21.965 µL | 109.827 µL | 219.655 µL | 1.098 mL | 2.197 mL |
10 mM | 10.983 µL | 54.914 µL | 109.827 µL | 549.137 µL | 1.098 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. SO Green™ 520WS Singlet Oxygen Sensor does not show any appreciable response to hydroxyl radical, superoxide, or other reactive oxygen species (ROS). This indicator emits a strong green fluorescence (excitation/emission maxima ∼504/525 nm) upon reaction with singlet oxygen. The PET quencher of SO Green™ 520WS Singlet Oxygen Sensor is eliminated (by singlet oxygen reaction) to recover its fluorescence.
References
View all 11 references: Citation Explorer
Desulfitative Sonogashira cross-coupling of thiopyronin for the synthesis of NIR arylacetylene-containing rhodamines.
Authors: Zhou, Guangshuai and Finney, Nathaniel and Wang, Yali
Journal: Chemical communications (Cambridge, England) (2024): 3039-3042
Authors: Zhou, Guangshuai and Finney, Nathaniel and Wang, Yali
Journal: Chemical communications (Cambridge, England) (2024): 3039-3042
The detection sensitivity of commonly used singlet oxygen probes in aqueous environments.
Authors: Entradas, Tiago and Waldron, Sarah and Volk, Martin
Journal: Journal of photochemistry and photobiology. B, Biology (2020): 111787
Authors: Entradas, Tiago and Waldron, Sarah and Volk, Martin
Journal: Journal of photochemistry and photobiology. B, Biology (2020): 111787
Polylysine modified conjugated polymer nanoparticles loaded with the singlet oxygen probe 1,3-diphenylisobenzofuran and the photosensitizer indocyanine green for use in fluorometric sensing and in photodynamic therapy.
Authors: Wang, Xiao-Hui and Yu, Yin-Xiao and Cheng, Kun and Yang, Wei and Liu, Yuan-An and Peng, Hong-Shang
Journal: Mikrochimica acta (2019): 842
Authors: Wang, Xiao-Hui and Yu, Yin-Xiao and Cheng, Kun and Yang, Wei and Liu, Yuan-An and Peng, Hong-Shang
Journal: Mikrochimica acta (2019): 842
NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen.
Authors: Ruiz-González, Rubén and Bresolí-Obach, Roger and Gulías, Òscar and Agut, Montserrat and Savoie, Huguette and Boyle, Ross W and Nonell, Santi and Giuntini, Francesca
Journal: Angewandte Chemie (International ed. in English) (2017): 2885-2888
Authors: Ruiz-González, Rubén and Bresolí-Obach, Roger and Gulías, Òscar and Agut, Montserrat and Savoie, Huguette and Boyle, Ross W and Nonell, Santi and Giuntini, Francesca
Journal: Angewandte Chemie (International ed. in English) (2017): 2885-2888
Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy.
Authors: Penon, Oriol and Marín, María J and Russell, David A and Pérez-García, Lluïsa
Journal: Journal of colloid and interface science (2017): 100-110
Authors: Penon, Oriol and Marín, María J and Russell, David A and Pérez-García, Lluïsa
Journal: Journal of colloid and interface science (2017): 100-110
Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: the misleading role of electron transfer.
Authors: Nardi, Giacomo and Manet, Ilse and Monti, Sandra and Miranda, Miguel A and Lhiaubet-Vallet, Virginie
Journal: Free radical biology & medicine (2014): 64-70
Authors: Nardi, Giacomo and Manet, Ilse and Monti, Sandra and Miranda, Miguel A and Lhiaubet-Vallet, Virginie
Journal: Free radical biology & medicine (2014): 64-70
Microheterogeneous concentrations of singlet oxygen in natural organic matter isolate solutions.
Authors: Grandbois, Matthew and Latch, Douglas E and McNeill, Kristopher
Journal: Environmental science & technology (2008): 9184-90
Authors: Grandbois, Matthew and Latch, Douglas E and McNeill, Kristopher
Journal: Environmental science & technology (2008): 9184-90
Oxidative damage to cellular and isolated DNA by homocysteine: implications for carcinogenesis.
Authors: Oikawa, Shinji and Murakami, Katsuhiko and Kawanishi, Shosuke
Journal: Oncogene (2003): 3530-8
Authors: Oikawa, Shinji and Murakami, Katsuhiko and Kawanishi, Shosuke
Journal: Oncogene (2003): 3530-8
Rational design of fluorescein-based fluorescence probes. Mechanism-based design of a maximum fluorescence probe for singlet oxygen.
Authors: Tanaka, K and Miura, T and Umezawa, N and Urano, Y and Kikuchi, K and Higuchi, T and Nagano, T
Journal: Journal of the American Chemical Society (2001): 2530-6
Authors: Tanaka, K and Miura, T and Umezawa, N and Urano, Y and Kikuchi, K and Higuchi, T and Nagano, T
Journal: Journal of the American Chemical Society (2001): 2530-6
A sensitive detection of neutrophil activation by fluorescence quenching of membrane inserted singlet oxygen probe.
Authors: Tanfani, F and Fiorini, R and Tartaglini, E and Kantar, A and Wozniak, M and Antosiewicz, J and Bertoli, E
Journal: Biochemistry and molecular biology international (1994): 1093-9
Authors: Tanfani, F and Fiorini, R and Tartaglini, E and Kantar, A and Wozniak, M and Antosiewicz, J and Bertoli, E
Journal: Biochemistry and molecular biology international (1994): 1093-9