Screen Quest™ CHO-Ga16- Chemokine (C-C) Receptor 2B Cells

Over 60% of the known G-protein-coupled receptors (GPCRs) signal through pathways other than Gq which lead to an increase in intracellular calcium. As genomics reveals more G-protein-coupled receptor targets this trend continues to increase. Screen Quest™ cell lines enable to investigate GPCR that do not conventionally couple through intracellular calcium. Screen Quest™ cell lines are based on a series of G-protein chimeras, including the promiscuous G-protein, Gα16. The chimeras consist of the alpha subunit of a Gq-protein complex whose 5 carboxy-terminal amino acids have been replaced with those from one of the other G-proteins (either Gαs, Gαi, Gαo, or Gαz). These amino acids are responsible for the coupling of the receptor to its G-protein. Co-expression of these chimeras with specific non-Gq-coupled receptors may result in the generation of an intracellular calcium signal upon receptor stimulation. Screen Quest™ CHO-Ga16- Chemokine (C-C) Receptor 2B (CHO-Ga16-CCR2B) cell line is CHO-K1 cells stably transfected with both the promiscuous G-protein, Gα16 and Chemokine (C-C) Receptor 2B receptor (CCR2B). The constitutively expressed Gα16 protein in the cells allows this receptor which normally act through the cAMP pathway, to couple to Gq signal transduction and mobilized intracellular calcium. Activation of the CCR2B in these cells by specific ligands can be detected using calcium sensitive dyes such as Calbryte 520 AM, Cal-520 AM, Fluo-8 AM, or Fluo-4 AM and the corresponding no wash calcium kits. This cell line has been successfully used in drug discovery and screening environments for studying GPCRs that do not conventionally couple through intracellular calcium. It has been effectively used with FLIPR and FDSS systems.

Page updated on July 12, 2023