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Rhod-5N, tripotassium salt
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-5N has a lower binding affinity for Ca2+ (Kd = ~320 µM) than any other BAPTA-based indicator and is suitable for Ca2+ measurements from 10 µM to 1 mM. Like the parent Rhod-2 indicator, Rhod-5N is essentially nonfluorescent in the absence of divalent cations and exhibits strong fluorescence enhancement with no spectral shift upon binding Ca2+.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Rhod-5N, tripotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 111.109 µL | 555.543 µL | 1.111 mL | 5.555 mL | 11.111 mL |
| 5 mM | 22.222 µL | 111.109 µL | 222.217 µL | 1.111 mL | 2.222 mL |
| 10 mM | 11.111 µL | 55.554 µL | 111.109 µL | 555.543 µL | 1.111 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Rhod-2, tripotassium salt | 553 | 577 | 0.11 |
| Rhod-FF, tripotassium salt | 553 | 577 | - |
Citations
View all 3 citations: Citation Explorer
Imaging Ca2+ Concentration and pH in Nanopores/Channels of Protein Crystals
Authors: Mori, Kazuo and Kuhn, Bernd
Journal: The Journal of Physical Chemistry B (2018): 9646--9653
Authors: Mori, Kazuo and Kuhn, Bernd
Journal: The Journal of Physical Chemistry B (2018): 9646--9653
Activation of mitochondrial transient receptor potential vanilloid 1 channel contributes to microglial migration
Authors: Miyake, Takahito and Shirakawa, Hisashi and Nakagawa, Takayuki and Kaneko, Shuji
Journal: Glia (2015): 1870--1882
Authors: Miyake, Takahito and Shirakawa, Hisashi and Nakagawa, Takayuki and Kaneko, Shuji
Journal: Glia (2015): 1870--1882
ER stress in human hepatic cells treated with Efavirenz: mitochondria again
Authors: Apostolova, Nadezda and Gomez-Sucerquia, Leysa J and Alegre, Fern and o , undefined and Funes, Haryes A and Victor, Victor M and Barrachina, Maria D and Blas-Garcia, Ana and Esplugues, Juan V
Journal: Journal of hepatology (2013): 780--789
Authors: Apostolova, Nadezda and Gomez-Sucerquia, Leysa J and Alegre, Fern and o , undefined and Funes, Haryes A and Victor, Victor M and Barrachina, Maria D and Blas-Garcia, Ana and Esplugues, Juan V
Journal: Journal of hepatology (2013): 780--789
References
View all 2 references: Citation Explorer
Ionic calcium determination in skim milk with molecular probes and front-face fluorescence spectroscopy: simple linear regression
Authors: Gangidi RR, Metzger LE.
Journal: J Dairy Sci (2006): 4105
Authors: Gangidi RR, Metzger LE.
Journal: J Dairy Sci (2006): 4105
Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N
Authors: McNamara CJ, Perry TDt, Bearce K, Hern and ez-Duque G, Mitchell R.
Journal: J Microbiol Methods (2005): 245
Authors: McNamara CJ, Perry TDt, Bearce K, Hern and ez-Duque G, Mitchell R.
Journal: J Microbiol Methods (2005): 245
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