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RH 237 [N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt]

RH 237, also called N-(4-sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, is a neuron-tracing dye. It is used for monitoring membrane potential, synaptic activity and ion channel activity of neurons.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of RH 237 [N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.329 µL1.007 mL2.013 mL10.066 mL20.133 mL
5 mM40.266 µL201.329 µL402.658 µL2.013 mL4.027 mL
10 mM20.133 µL100.664 µL201.329 µL1.007 mL2.013 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Citations

View all 6 citations: Citation Explorer
The effect of sex and age on ex vivo cardiac electrophysiology: insight from a guinea pig model
Authors: Haq, Kazi T and Cooper, Blake L and Berk, Fiona and Posnack, Nikki G
Journal: American Journal of Physiology-Heart and Circulatory Physiology (2022)
Optocardiography and electrophysiology studies of ex vivo langendorff-perfused hearts
Authors: Swift, Luther M and Jaimes III, Rafael and McCullough, Damon and Burke, Morgan and Reilly, Marissa and Maeda, Takuya and Zhang, Hanyu and Ishibashi, Nobuyuki and Rogers, Jack M and Posnack, Nikki Gillum
Journal: Journal of visualized experiments: JoVE (2019)
Translational Physiology: Investigating the utility of adult zebrafish ex vivo whole hearts to pharmacologically screen hERG channel activator compounds
Authors: Hull, Christina M and Genge, Christine E and Hobbs, Yuki and Rayani, Kaveh and Lin, Eric and Gunawan, Marvin and Shafaattalab, Sanam and Tibbits, Glen F and Claydon, Tom W
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2019): R921
Investigating the utility of adult zebrafish ex vivo whole hearts to pharmacologically screen hERG channel activator compounds
Authors: Hull, Christina M and Genge, Christine E and Hobbs, Yuki and Rayani, Kaveh and Lin, Eric and Gunawan, Marvin and Shafaattalab, Sanam and Tibbits, Glen F and Claydon, Tom W
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2019): R921--R931
Age-associated changes in electrical function of the zebrafish heart
Authors: Stoyek, Matthew R and Rog-Zielinska, Eva A and Quinn, T Alex and er, undefined
Journal: Progress in biophysics and molecular biology (2018)

References

View all 11 references: Citation Explorer
Simultaneous optical imaging of membrane potential and intracellular calcium
Authors: Fast VG., undefined
Journal: J Electrocardiol (2005): 107
Contrasting effects of ischemia on the kinetics of membrane voltage and intracellular calcium transient underlie electrical alternans
Authors: Lakireddy V, Baweja P, Syed A, Bub G, Boutjdir M, El-Sherif N.
Journal: Am J Physiol Heart Circ Physiol (2005): H400
Second-harmonic generation sensitivity to transmembrane potential in normal and tumor cells
Authors: Sacconi L, D'Amico M, Vanzi F, Biagiotti T, Antolini R, Olivotto M, Pavone FS.
Journal: J Biomed Opt (2005): 24014
Intracellular Ca dynamics in ventricular fibrillation
Authors: Omichi C, Lamp ST, Lin SF, Yang J, Baher A, Zhou S, Attin M, Lee MH, Karagueuzian HS, Kogan B, Qu Z, Garfinkel A, Chen PS, Weiss JN.
Journal: Am J Physiol Heart Circ Physiol (2004): H1836
Effects of electrical shocks on Cai2+ and Vm in myocyte cultures
Authors: Fast VG, Cheek ER, Pollard AE, Ideker RE.
Journal: Circ Res (2004): 1589
Page updated on November 23, 2024

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Catalog Number21480
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Physical properties

Molecular weight

496.7

Solvent

DMSO

Spectral properties

Excitation (nm)

550

Emission (nm)

786

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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