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ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 466.54 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Related products
| Overview |  SDSProtocol |
See also: Buffers and Lab Consumables
CAS 62996-74 | Molecular weight 466.54 |
Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases, e.g., CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiUse™ Staurosporine * 1 mM DMSO stock solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 214.344 µL | 1.072 mL | 2.143 mL | 10.717 mL | 21.434 mL |
| 5 mM | 42.869 µL | 214.344 µL | 428.688 µL | 2.143 mL | 4.287 mL |
| 10 mM | 21.434 µL | 107.172 µL | 214.344 µL | 1.072 mL | 2.143 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) |
| ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* | 498 | 517 |
| ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free* | 565 | 574 |
Images
Figure 1. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Figure 2. Chemical structure for ReadiUse™ Staurosporine * 1 mM DMSO stock solution*.
Citations
View all 35 citations: Citation Explorer
Determination of the optical interference of iron oxide nanoparticles in fluorometric cytotoxicity assays
Authors: Martin, Leisha and Lopez, Kimberly and Fritz, Shayden and Easterling, Charles P and Krawchuck, Jacob A and Poerwoprajitno, Agus R and Xu, Wei
Journal: Heliyon (2024): e25378
Authors: Martin, Leisha and Lopez, Kimberly and Fritz, Shayden and Easterling, Charles P and Krawchuck, Jacob A and Poerwoprajitno, Agus R and Xu, Wei
Journal: Heliyon (2024): e25378
Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells
Authors: Glassmann, A., Carrillo Garcia, C., Janzen, V., Kraus, D., Veit, N., Winter, J., Probstmeier, R.
Journal: Anal Cell Pathol (Amst) (2018): 1754085
Authors: Glassmann, A., Carrillo Garcia, C., Janzen, V., Kraus, D., Veit, N., Winter, J., Probstmeier, R.
Journal: Anal Cell Pathol (Amst) (2018): 1754085
Bioactive staurosporine derivatives from the Streptomyces sp. NB-A13
Authors: Zhou, B., Hu, Z. J., Zhang, H. J., Li, J. Q., Ding, W. J., Ma, Z. J.
Journal: Bioorg Chem (2018): 33-40
Authors: Zhou, B., Hu, Z. J., Zhang, H. J., Li, J. Q., Ding, W. J., Ma, Z. J.
Journal: Bioorg Chem (2018): 33-40
7-hydroxy-staurosporine, UCN-01, induces DNA damage response, and autophagy in human osteosarcoma U2-OS cells
Authors: Lien, W. C., Chen, T. Y., Sheu, S. Y., Lin, T. C., Kang, F. C., Yu, C. H., Kuan, T. S., Huang, B. M., Wang, C. Y.
Journal: J Cell Biochem (2018): 4729-4741
Authors: Lien, W. C., Chen, T. Y., Sheu, S. Y., Lin, T. C., Kang, F. C., Yu, C. H., Kuan, T. S., Huang, B. M., Wang, C. Y.
Journal: J Cell Biochem (2018): 4729-4741
Raman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporine
Authors: Jamieson, L. E., Harrison, D. J., Campbell, C. J.
Journal: J Biophotonics (2018): e201800201
Authors: Jamieson, L. E., Harrison, D. J., Campbell, C. J.
Journal: J Biophotonics (2018): e201800201
Staurosporine, an inhibitor of hormonally up-regulated neu-associated kinase
Authors: Zambrano, J. N., Williams, C. J., Williams, C. B., Hedgepeth, L., Burger, P., Dilday, T., Eblen, S. T., Armeson, K., Hill, E. G., Yeh, E. S.
Journal: Oncotarget (2018): 35962-35973
Authors: Zambrano, J. N., Williams, C. J., Williams, C. B., Hedgepeth, L., Burger, P., Dilday, T., Eblen, S. T., Armeson, K., Hill, E. G., Yeh, E. S.
Journal: Oncotarget (2018): 35962-35973
Ampakines Attenuate Staurosporine-induced Cell Death in Primary Cortical Neurons: Implications in the 'Chemo-Brain' Phenomenon
Authors: Radin, D. P., Rogers, G. A., Hewitt, K. E., Purcell, R., Lippa, A.
Journal: Anticancer Res (2018): 3461-3465
Authors: Radin, D. P., Rogers, G. A., Hewitt, K. E., Purcell, R., Lippa, A.
Journal: Anticancer Res (2018): 3461-3465
Encephalitozoon cuniculi and Vittaforma corneae (Phylum Microsporidia) inhibit staurosporine-induced apoptosis in human THP-1 macrophages in vitro
Authors: Sokolova, Y. Y., Bowers, L. C., Alvarez, X., Didier, E. S.
Journal: Parasitology (2018): 1-11
Authors: Sokolova, Y. Y., Bowers, L. C., Alvarez, X., Didier, E. S.
Journal: Parasitology (2018): 1-11
Changes in the Biophysical Properties of the Cell Membrane Are Involved in the Response of Neurospora crassa to Staurosporine
Authors: Santos, F. C., Lobo, G. M., Fern and es, A. S., Videira, A., de Almeida, R. F. M.
Journal: Front Physiol (2018): 1375
Authors: Santos, F. C., Lobo, G. M., Fern and es, A. S., Videira, A., de Almeida, R. F. M.
Journal: Front Physiol (2018): 1375
The ubiquitin E3 ligase CHIP promotes proteasomal degradation of the serine/threonine protein kinase PINK1 during staurosporine-induced cell death
Authors: Yoo, L., Chung, K. C.
Journal: J Biol Chem (2018): 1286-1297
Authors: Yoo, L., Chung, K. C.
Journal: J Biol Chem (2018): 1286-1297