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ReadiUse™ RBC Lysis Buffer
Product key features
- Ready-to-Use: Pre-formulated 1X solution eliminates the need for dilution or preparation.
- Selective Erythrocyte Lysis: Efficiently lyses red blood cells while preserving leukocyte viability and function.
- Optimized for Single-Cell Suspensions – Ideal for mouse hematopoietic tissues and human peripheral blood.
- Minimal Impact on Leukocytes: Designed to minimize alterations in lymphocyte integrity for downstream applications.
- Reproducible and Reliable: Rigorously validated for consistent RBC removal in immunology, hematology, and flow cytometry workflows.
Product description
ReadiUse™ RBC Lysis Buffer is a ready-to-use 1X solution optimized for the selective lysis of red blood cells (RBCs) in single-cell suspensions while preserving the viability and functionality of leukocytes. This buffer is specifically designed for processing mouse hematopoietic tissues, such as spleen, and human peripheral blood samples, making it suitable for a wide range of immunology, hematology, and flow cytometry applications.
The formulation contains ammonium chloride, which induces osmotic lysis of erythrocytes while exerting minimal effects on lymphocytes and other nucleated white blood cells when used as recommended. However, nucleated red blood cells are not effectively lysed by ammonium chloride and may require alternative or additional treatment.
ReadiUse™ RBC Lysis Buffer is supplied as a pre-diluted, ready-to-use solution, eliminating the need for preparation steps and ensuring reproducibility across experiments. This reagent has been rigorously tested to provide efficient RBC removal with minimal leukocyte loss, making it an ideal choice for downstream applications requiring high-purity immune cell populations.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
- Warm the ReadiUse™ RBC Lysis Buffer to room temperature prior to use.
- Take upto 100 µL of whole blood and add 2 mL of ReadiUse™ RBC Lysis Buffer.
- Vortex this tube gently.
- Incubate at room temperature (in a dark place) for 10-15 minutes.
- Centrifuge at 350g for 5 mins. Discard the supernatant without disturbing the pellet.
- Wash the pellet by resuspending it in appropriate buffer of your choice, and re-centrifuge.
- Resuspend the pellet and proceed further with experiment.
- Harvest the spleen and prepare a single cell suspension.
- Centrifuge at 350g for 5 mins to pellet the cells, discard the supernatant.
- Resuspend the pellet in 5mL of ReadiUse™ RBC Lysis Buffer.
- Incubate on ice for 5 minutes with intermittent tapping/shaking.
- Dilute this reaction mixture by adding 25mL of 1X PBS. Note: This volume can be adjusted based on need (20-30mL).
- Centrifuge at 350g for 5 mins. Discard the supernatant without disturbing the pellet.
- Wash the pellet by resuspending it in appropriate buffer of your choice, and re-centrifuge.
- Resuspend the pellet and proceed further with experiment.
References
Authors: Lee, Kiara and Tripathi, Anubhav
Journal: Biopreservation and biobanking (2024): 130-138
Authors: Seghers, Sofie and Le Compte, Maxim and Hendriks, Jeroen M H and Van Schil, Paul and Janssens, Annelies and Wener, Reinier and Komen, Niels and Prenen, Hans and Deben, Christophe
Journal: Critical reviews in oncology/hematology (2024): 104285
Authors: Buyl, Karolien and Merimi, Makram and Rodrigues, Robim M and Rahmani, Saida and Fayyad-Kazan, Mohammad and Bouhtit, Fatima and Boukhatem, Noureddine and Vanhaecke, Tamara and Fahmi, Hassan and De Kock, Joery and Najar, Mehdi
Journal: Biomolecules (2024)
Authors: Zhao, Wenxiu and Yu, Haibo and Ge, Zhixing and Wang, Xiaoduo and Zhang, Yuzhao and Wen, Yangdong and Luo, Hao and Liu, Lianqing and Li, Wen Jung
Journal: Lab on a chip (2022): 727-732
Authors: Govindarajalu, Gokulapriya and Rizvi, Zeba and Kumar, Deepak and Sijwali, Puran Singh
Journal: Scientific reports (2019): 19952
Product protocol