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ReadiUse™ 10KD Spin Filter
ReadiUse™ 10KD Spin Filter is a disposable ultrafiltration device for the concentration of biological samples such as antibodies. Maximum initial sample volume is ~500 uL. It can be used in either a swing bucket or fixed angle rotors accepting 2.0 mL centrifuge tube at around 10000 g. The dead stop volume is ~20-25 µL. The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area to achieve superior recovery from dilute solutions.
Example protocol
AT A GLANCE
Overview
- Polypropylene housing and polyethersulfone (PES) membrane
- MWCO: 10K
- Sample Volume: 25 µL-500 µL
- High sample recovery
- Application: Protein concentration, desalting, buffer exchange, and deproteinization of biological samples
- Maximum Centrifugal Force: 14,000 g
- Recommended Centrifugal Force: 5,000 ∼ 10,000 g
SAMPLE EXPERIMENTAL PROTOCOL
Concentrate volume
Add protein sample to the filter, centrifuge at 8,000 ∼ 10,000 g.Note it takes about 10 ∼ 15 mins to concentrate from 500 µL to ~50 µL.
Desalting
Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the ReadiUse™ 10KD Spin Filter by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced.Deproteinization of biological samples
Add cell lysates or tissue homogenates to the filter, centrifuge at ∼10,000 g for 10 ∼ 15min, collect the elution.References
View all 50 references: Citation Explorer
Extraction of β-blockers from urine with a polymeric monolith modified with 1-allyl-3-methylimidazolium chloride in spin column format.
Authors: Mompó-Roselló, Oscar and Ribera-Castelló, Ana and Simó-Alfonso, Ernesto F and Ruiz-Angel, María José and García-Alvarez-Coque, María Celia and Herrero-Martínez, José Manuel
Journal: Talanta (2020): 120860
Authors: Mompó-Roselló, Oscar and Ribera-Castelló, Ana and Simó-Alfonso, Ernesto F and Ruiz-Angel, María José and García-Alvarez-Coque, María Celia and Herrero-Martínez, José Manuel
Journal: Talanta (2020): 120860
Spin-column micro-solid phase extraction of chlorophenols using MFU-4l metal-organic framework.
Authors: Esrafili, Ali and Ghambarian, Mahnaz and Tajik, Mohammad and Baharfar, Mahroo
Journal: Mikrochimica acta (2019): 39
Authors: Esrafili, Ali and Ghambarian, Mahnaz and Tajik, Mohammad and Baharfar, Mahroo
Journal: Mikrochimica acta (2019): 39
Synthesis and characterization of polyamide-graphene oxide-polypyrrole electrospun nanofibers for spin-column micro solid phase extraction of parabens in milk samples.
Authors: Seidi, Shahram and Sadat Karimi, Elnaz and Rouhollahi, Ahmad and Baharfar, Mahroo and Shanehsaz, Maryam and Tajik, Mohammad
Journal: Journal of chromatography. A (2019): 25-34
Authors: Seidi, Shahram and Sadat Karimi, Elnaz and Rouhollahi, Ahmad and Baharfar, Mahroo and Shanehsaz, Maryam and Tajik, Mohammad
Journal: Journal of chromatography. A (2019): 25-34
Filter paper-based spin column method for cost-efficient DNA or RNA purification.
Authors: Shi, Rui and Lewis, Ramsey S and Panthee, Dilip R
Journal: PloS one (2018): e0203011
Authors: Shi, Rui and Lewis, Ramsey S and Panthee, Dilip R
Journal: PloS one (2018): e0203011
A spin column-free approach to sodium hydroxide-based glycan permethylation.
Authors: Hu, Yueming and Borges, Chad R
Journal: The Analyst (2017): 2748-2759
Authors: Hu, Yueming and Borges, Chad R
Journal: The Analyst (2017): 2748-2759
Page updated on November 21, 2024
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