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Probenecid *Cell culture tested* *CAS 57-66-9*
Probenecid is an inhibitor of organic-anion transporters located in cell membranes. These transporters often extrude fluorescent indicators from cells, and therefore contribute to poor dye retention. This phenomenon usually causes high background in the assays that require the good retention of the dye indicators inside cells. The use of probenecid to inhibit the transporter activity, and thus to reduce leakage of the intracellular dye indicators is a common method for reducing fluorescence background of calcium assays. Each vial is dissovled in 0.3 mL of 1 M NaOH, and then diluted into 100 mL buffer to make 2.5 mM final concentration of probenecid for your assays.
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Common stock solution preparation
Table 1. Volume of 1 M NaOH needed to reconstitute specific mass of Probenecid *Cell culture tested* *CAS 57-66-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 350.435 µL | 1.752 mL | 3.504 mL | 17.522 mL | 35.043 mL |
| 5 mM | 70.087 µL | 350.435 µL | 700.869 µL | 3.504 mL | 7.009 mL |
| 10 mM | 35.043 µL | 175.217 µL | 350.435 µL | 1.752 mL | 3.504 mL |
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Citations
View all 13 citations: Citation Explorer
Magnetic-field-synchronized wireless modulation of neural activity by magnetoelectric nanoparticles
Authors: Zhang, E and Abdel-Mottaleb, M and Liang, P and Navarrete, B and Yildirim, Y Akin and Campos, M Alberteris and Smith, IT and Wang, P and Yildirim, B and Yang, L and others,
Journal: Brain Stimulation (2022): 1451--1462
Authors: Zhang, E and Abdel-Mottaleb, M and Liang, P and Navarrete, B and Yildirim, Y Akin and Campos, M Alberteris and Smith, IT and Wang, P and Yildirim, B and Yang, L and others,
Journal: Brain Stimulation (2022): 1451--1462
Pharmacogenomic screening of drug candidates using patient-specific hiPSC-derived cardiomyocyte high-throughput calcium imaging
Authors: Blancard, Malorie and Fetterman, K Ashley and Burridge, Paul W
Journal: (2022): 241--253
Authors: Blancard, Malorie and Fetterman, K Ashley and Burridge, Paul W
Journal: (2022): 241--253
Identification of the material basis of the medicinal properties in Curcuma Longa L. to enhance targeted clinical application
Authors: Peng, Sha and Li, Jing and Huo, Mengqi and Cao, Yanfeng and Chen, Zhao and Zhang, Yanling and Qiao, Yanjiang
Journal: Journal of Traditional Chinese Medical Sciences (2022): 374--382
Authors: Peng, Sha and Li, Jing and Huo, Mengqi and Cao, Yanfeng and Chen, Zhao and Zhang, Yanling and Qiao, Yanjiang
Journal: Journal of Traditional Chinese Medical Sciences (2022): 374--382
Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
Authors: Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun
Journal: BioTechniques (2021): 271--277
Authors: Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun
Journal: BioTechniques (2021): 271--277
References
View all 35 references: Citation Explorer
DNA damage caused by benzo(a)pyrene in MCF-7 cells is increased by verapamil, probenecid and PSC833
Authors: Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K.
Journal: Toxicol Lett (2007): 3
Authors: Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K.
Journal: Toxicol Lett (2007): 3
Is the monkey an appropriate animal model to examine drug-drug interactions involving renal clearance? Effect of probenecid on the renal elimination of H2 receptor antagonists
Authors: Tahara H, Kusuhara H, Chida M, Fuse E, Sugiyama Y.
Journal: J Pharmacol Exp Ther (2006): 1187
Authors: Tahara H, Kusuhara H, Chida M, Fuse E, Sugiyama Y.
Journal: J Pharmacol Exp Ther (2006): 1187
Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid
Authors: Tahara H, Kusuhara H, Maeda K, Koepsell H, Fuse E, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 743
Authors: Tahara H, Kusuhara H, Maeda K, Koepsell H, Fuse E, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 743
MRP2 (ABCC2) transports taxanes and confers paclitaxel resistance and both processes are stimulated by probenecid
Authors: Huisman MT, Chhatta AA, van Tellingen O, Beijnen JH, Schinkel AH.
Journal: Int J Cancer (2005): 824
Authors: Huisman MT, Chhatta AA, van Tellingen O, Beijnen JH, Schinkel AH.
Journal: Int J Cancer (2005): 824
Mediation of annexin 1 secretion by a probenecid-sensitive ABC-transporter in rat inflamed mucosa
Authors: Wein S, Fauroux M, Laffitte J, de Nadai P, Guaini C, Pons F, Comera C.
Journal: Biochem Pharmacol (2004): 1195
Authors: Wein S, Fauroux M, Laffitte J, de Nadai P, Guaini C, Pons F, Comera C.
Journal: Biochem Pharmacol (2004): 1195
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