Phen Green™ SK, Diacetate

• Broad metal ion selectivity – Detects key physiological and toxic metal ions, including Fe²⁺, Cu⁺/²⁺, Zn²⁺, Cd²⁺, Ni²⁺, Co²⁺, Hg²⁺, and Pb²⁺.
• Quantitative fluorescence quenching – Enables sensitive, concentration-dependent detection through metal-induced fluorescence attenuation.
• Live-cell suitability – Cell-permeable design supports dynamic monitoring of labile intracellular metal ion pools in real time.
• Esterase-mediated activation – Intracellular esterases hydrolyze the diacetate form, releasing the active fluorophore with minimal background fluorescence.
• Versatile assay compatibility – Optimized for fluorescence microscopy and high-throughput microplate-based detection platforms.

Phen Green™ SK, Diacetate is a cell-permeable, fluorogenic chelator designed for the sensitive detection of intracellular transition and heavy metal ions in live-cell applications. Upon cellular uptake, cytosolic esterases efficiently hydrolyze the diacetate protecting groups, yielding the highly fluorescent, metal-sensitive Phen Green™ SK dye. The activated probe exhibits excitation and emission maxima at approximately 507 nm and 532 nm, respectively, and undergoes fluorescence quenching in the presence of a broad range of divalent and monovalent metal ions, including Fe²⁺, Cu²⁺, Cu⁺, Zn²⁺, Cd²⁺, Ni²⁺, Co²⁺, Hg²⁺, and Pb²⁺.

This metal-induced quenching mechanism allows for direct, concentration-dependent monitoring of labile metal pools within the cytosol. Phen Green™ SK, Diacetate is well-suited for use in both fluorescence microscopy and high-throughput microplate assays, offering flexibility across experimental platforms. Its broad metal ion reactivity and robust signal response make it a powerful tool for investigating intracellular metal ion dynamics, redox regulation, metal transport mechanisms, and metal-associated cytotoxicity in diverse biological systems.