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Phycoerythrin (PE)

R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The relative prominence of the secondary peaks varies significantly among R-PEs from different species. PE has three types of subunits: alpha (20,000 daltons), beta (20,000 daltons) and gamma (30,000 daltons). The molecular weight of intact PE has been found to be about 240,000 daltons. The alpha subunit of PE contains only the phycoerythrobilin (PEB) chromophore, while beta and gamma subunits contain both PEB and phycourobilin (PUB). Variability in the absorption spectra of PEs from various species reflects differences in the PEB/PUB ratio of the subunits. PE and closely related B-PE are the most intensely fluorescent phycobiliproteins, with quantum efficiencies probably in excess of 90%, and its orange fluorescence is readily visible by eye in any moderately concentrated solution.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*56557419600000.820.175

Citations

View all 6 citations: Citation Explorer
The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature
Authors: Sakamoto, Yasuhisa and Uezu, Akiyoshi and Kikuchi, Koji and Kang, Jangmi and Fujii, Eiko and Moroishi, Toshiro and Suetsugu, Shiro and Nakanishi, Hiroyuki
Journal: The EMBO Journal (2024): 1--27
Targeting pro-inflammatory T cells as a novel therapeutic approach to potentially resolve atherosclerosis in humans
Authors: Fan, Lin and Liu, Junwei and Hu, Wei and Chen, Zexin and Lan, Jie and Zhang, Tongtong and Zhang, Yang and Wu, Xianpeng and Zhong, Zhiwei and Zhang, Danyang and others,
Journal: Cell Research (2024): 1--21
Direct Cytosolic Delivery of Proteins and CRISPR-Cas9 Genome Editing by Gemini Amphiphiles via Non-Endocytic Translocation Pathways
Authors: Le, Zhicheng and Pan, Qi and He, Zepeng and Liu, Hong and Shi, Yi and Liu, Lixin and Liu, Zhijia and Ping, Yuan and Chen, Yongming
Journal: ACS Central Science (2023)
CD169+ subcapsular sinus macrophage-derived microvesicles are associated with light zone follicular dendritic cells
Authors: Chen, Xin and Zheng, Yuhan and Liu, Siming and Yu, Wenjing and Liu, Zhiduo
Journal: European Journal of Immunology (2022): 1581--1594
Nanobubbles Containing sPD-1 and Ce6 Mediate Combination Immunotherapy and Suppress Hepatocellular Carcinoma in Mice
Authors: Tan, Yandi and Yang, Shiqi and Ma, Yao and Li, Jinlin and Xie, Qian and Liu, Chaoqi and Zhao, Yun
Journal: International Journal of Nanomedicine (2021): 3241

References

View all 46 references: Citation Explorer
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Page updated on October 28, 2024

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Physical properties

Molecular weight

~240000

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.175

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

574

Quantum yield

0.82

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501

CAS

11016-17-4
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE specific B6-A channel.
Flow cytometry analysis of whole blood stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE specific B4-A channel.
Phycoerythrin&nbsp;(PE) is a red protein-pigment&nbsp;complex from the light harvesting&nbsp;phycobiliprotein&nbsp;family, present in&nbsp;red algae&nbsp;and&nbsp;cryptophytes,&nbsp;accessory to the main&nbsp;chlorophyll&nbsp;pigments responsible for&nbsp;photosynthesis. Like all phycobiliproteins, it is composed of a protein part covalently binding&nbsp;chromophores&nbsp;called&nbsp;phycobilins. In the phycoerythrin family, the most known phycobilins are:&nbsp;phycoerythrobilin, the typical phycoerythrin acceptor chromophore, and sometimes&nbsp;phycourobilin. Phycoerythrins are composed of (&alpha;&beta;) monomers, usually organized in a disk-shaped&nbsp;trimer&nbsp;(&alpha;&beta;)<sub>3</sub>&nbsp;or&nbsp;hexamer&nbsp;(&alpha;&beta;)<sub>6</sub>&nbsp;(second one is the functional unit of the&nbsp;antenna rods). These typical complexes also contain a third type of subunit, the &gamma; chain. Phycobilin pigments have excellent fluorescent properties that are extremely useful for flow cytometry-based immunoassays.

Documents

pdfCertificate of origin
pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
PE and APC
Spectral Flow Cytometry