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OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant*
OG488 BAPTA -1 is the same molecule of Oregon Green 488 BAPTA-1. It is a visible light-excitable calcium indicator that is often used with FITC filter set. OG 488 BAPTA-1 has excitation and emission at 494 and 523 nm respectively. Its fluorescence intensity increases by ~14 fold upon binding calcium ion. This water-soluble, salt form is useful for intracellular loading by microinjection or infusion from patch pipette. The cell-permeant AM ester form of the indicator is also available (#20507).
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 89.744 µL | 448.72 µL | 897.44 µL | 4.487 mL | 8.974 mL |
| 5 mM | 17.949 µL | 89.744 µL | 179.488 µL | 897.44 µL | 1.795 mL |
| 10 mM | 8.974 µL | 44.872 µL | 89.744 µL | 448.72 µL | 897.44 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 38 references: Citation Explorer
Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18
Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61
Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243
Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477
Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339
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