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MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

A fluorogenic substrate for phosphatases. It is widely used for detecting phosphatases in solution. However this phosphatase substrate is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer MUP Plus that is developed to address this pH limitation associated with MUP substrates.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM333.211 µL1.666 mL3.332 mL16.661 mL33.321 mL
5 mM66.642 µL333.211 µL666.422 µL3.332 mL6.664 mL
10 mM33.321 µL166.606 µL333.211 µL1.666 mL3.332 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Fluorescein, disodium salt *CAS 518-47-8*4985178000010.79001, 0.9520.320.35

References

View all 76 references: Citation Explorer
Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Relative movement and soil fixation of soluble organic and inorganic phosphorus
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Page updated on December 3, 2024

Ordering information

Price
Unit size
25 mg
10 g
Catalog Number
1161011612
Quantity
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Physical properties

Molecular weight

300.11

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.1

Extinction coefficient (cm -1 M -1)

18000

Excitation (nm)

360

Emission (nm)

448

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

22919-26-2
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