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mFluor™ Violet 550-streptavidin conjugate

mFluor™ Violet 550-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 550 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 550 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor ™ Violet 550 exhibits a bright and photostable emission of green fluorescence at 550 nm. mFluor™ Violet 550 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate4064453500010.8110.3380.078
mFluor™ Violet 510-streptavidin conjugate4125052500010.8610.4640.366
mFluor™ Violet 540-streptavidin conjugate4025351800010.2111.3260.543
mFluor™ Violet 545-streptavidin conjugate3935432000010.1511.080.496
mFluor™ Violet 590-streptavidin conjugate5645919000010.2210.6320.329
mFluor™ Violet 610-streptavidin conjugate5946129000010.310.5320.66
Page updated on November 23, 2024

Ordering information

Price
Unit size
Catalog Number47252
Quantity
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Additional ordering information

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Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

~52 kDa

Solvent

Water

Spectral properties

Absorbance (nm)

527

Correction Factor (260 nm)

0.474

Correction Factor (280 nm)

0.306

Extinction coefficient (cm -1 M -1)

900001

Excitation (nm)

527

Emission (nm)

550

Quantum yield

0.311

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200