mFluor™ Violet 545-streptavidin conjugate

The mFluor™ Violet 545-streptavidin conjugate is a tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 545 fluorescent dyes. It has a strong binding affinity for biotin conjugates, making it useful in various biochemical applications for detecting specific proteins, protein motifs, nucleic acids, and other molecules. This concept is essential for workflows such as western blots, flow cytometry, imaging and microscopy, and microplate assays, as well as for purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 545 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. When excited by a violet laser, mFluor™ Violet 545 emits bright and photostable green fluorescence at 543 nm. mFluor™ Violet 545 streptavidin conjugates are particularly useful as second-step reagents for indirect immunofluorescent staining when used with biotinylated primary antibodies.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate	406	445	35000¹	0.81¹	0.338	0.078
mFluor™ Violet 510-streptavidin conjugate	412	505	25000¹	0.86¹	0.464	0.366
mFluor™ Violet 540-streptavidin conjugate	402	535	18000¹	0.21¹	1.326	0.543
mFluor™ Violet 550-streptavidin conjugate	527	550	90000¹	0.31¹	0.474	0.306
mFluor™ Violet 590-streptavidin conjugate	564	591	90000¹	0.22¹	0.632	0.329
mFluor™ Violet 610-streptavidin conjugate	594	612	90000¹	0.3¹	0.532	0.66

References

View all 22 references: Citation Explorer

Low Concentrations of Chlorhexidine Inhibit the Formation and Structural Integrity of Enzyme-Treated Multispecies Oral Biofilms.
Authors: Gränicher, Kay Andrin and Karygianni, Lamprini and Attin, Thomas and Thurnheer, Thomas
Journal: Frontiers in microbiology (2021): 741863

Protein immobilization onto electrochemically synthesized CoFe nanowires.
Authors: Torati, Sri Ramulu and Reddy, Venu and Yoon, Seok Soo and Kim, CheolGi
Journal: International journal of nanomedicine (2015): 645-51

In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.
Authors: Towner, Rheal A and Smith, Nataliya and Saunders, Debra and Henderson, Michael and Downum, Kristen and Lupu, Florea and Silasi-Mansat, Robert and Ramirez, Dario C and Gomez-Mejiba, Sandra E and Bonini, Marcelo G and Ehrenshaft, Marilyn and Mason, Ronald P
Journal: Diabetes (2012): 2405-13

Molecular MRI assessment of vascular endothelial growth factor receptor-2 in rat C6 gliomas.
Authors: He, Ting and Smith, Nataliya and Saunders, Debra and Doblas, Sabrina and Watanabe, Yasuko and Hoyle, Jessica and Silasi-Mansat, Robert and Lupu, Florea and Lerner, Megan and Brackett, Daniel J and Towner, Rheal A
Journal: Journal of cellular and molecular medicine (2011): 837-49

Sequence-dependent fluorescence of cyanine dyes on microarrays.
Authors: Agbavwe, Christy and Somoza, Mark M
Journal: PloS one (2011): e22177

Page updated on November 23, 2024

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Catalog Number	47250
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Physical properties

Molecular weight	~52 kDa
Solvent	Water

Spectral properties

Absorbance (nm)	398
Correction Factor (260 nm)	1.08
Correction Factor (280 nm)	0.496
Extinction coefficient (cm ^-1 M ^-1)	20000¹
Excitation (nm)	393
Emission (nm)	543
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200