mFluor™ Violet 510-streptavidin conjugate

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This mFluor™ Violet 510-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ Violet 510 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using a flow cytometer equiped with a 405 nm excitation (e.g., the Violet Laser). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate	406	445	35000¹	0.81¹	0.338	0.078
mFluor™ Violet 540-streptavidin conjugate	402	535	18000¹	0.21¹	1.326	0.543
mFluor™ Violet 545-streptavidin conjugate	393	543	20000¹	0.15¹	1.08	0.496
mFluor™ Violet 550-streptavidin conjugate	527	550	90000¹	0.31¹	0.474	0.306
mFluor™ Violet 590-streptavidin conjugate	564	591	90000¹	0.22¹	0.632	0.329
mFluor™ Violet 610-streptavidin conjugate	594	612	90000¹	0.3¹	0.532	0.66

Citations

View all 1 citations: Citation Explorer

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400

Page updated on November 23, 2024

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Catalog Number	16931
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Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Absorbance (nm)	410
Correction Factor (260 nm)	0.464
Correction Factor (280 nm)	0.366
Extinction coefficient (cm ^-1 M ^-1)	25000¹
Excitation (nm)	412
Emission (nm)	505
Quantum yield	0.86¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200