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mBBr [Monobromobimane] *CAS 71418-44-5*
mBBr is one of the most popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. mBBr is used for the determination of the redox status of low molecular weight and protein thiols in biological systems. MBBr-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and bromobimane accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy following protein precipitation.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mBBr [Monobromobimane] *CAS 71418-44-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 368.854 µL | 1.844 mL | 3.689 mL | 18.443 mL | 36.885 mL |
| 5 mM | 73.771 µL | 368.854 µL | 737.708 µL | 3.689 mL | 7.377 mL |
| 10 mM | 36.885 µL | 184.427 µL | 368.854 µL | 1.844 mL | 3.689 mL |
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Citations
View all 1 citations: Citation Explorer
The development of antioxidant system in the intestinal tract of broiler chickens
Authors: Tang, Dazhi and Wu, Jianmin and Jiao, Hongchao and Wang, Xiaojuan and Zhao, Jingpeng and Lin, Hai
Journal: Poultry science (2019): 664--678
Authors: Tang, Dazhi and Wu, Jianmin and Jiao, Hongchao and Wang, Xiaojuan and Zhao, Jingpeng and Lin, Hai
Journal: Poultry science (2019): 664--678
References
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Journal: AIDS Res Hum Retroviruses (2000): 1929
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Authors: Guthmann F, Haupt R, Looman AC, Spener F, Rustow B.
Journal: Am J Physiol (1999): L191
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