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hBSA *Optimized for Maximum Immunization Response*

hBSA is the bovine serum albumin modified with hexamethylenediamine to make the proteins bear more amine groups (thus more positive charges at neutral pH). It is proven to be a superior carrier protein for developing antibodies since cBSA conjugates have stronger imunological responses than the unmodifed BSA cinjugates.

Calculators

Common stock solution preparation

Table 1. Volume of appropriate solvent needed to reconstitute specific mass of hBSA *Optimized for Maximum Immunization Response* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM1.667 µL8.333 µL16.667 µL83.333 µL166.667 µL
5 mM333.333 nL1.667 µL3.333 µL16.667 µL33.333 µL
10 mM166.667 nL833.333 nL1.667 µL8.333 µL16.667 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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References

View all 29 references: Citation Explorer
Optimization of the preparation process of vinblastine sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM)
Authors: Zu Y, Zhang Y, Zhao X, Zhang Q, Liu Y, Jiang R.
Journal: Int J Nanomedicine (2009): 321
Characterization of bovine serum albumin glycated with glucose, galactose and lactose
Authors: Ledesma-Osuna AI, Ramos-Clamont G, Vazquez-Moreno L.
Journal: Acta Biochim Pol (2008): 491
Improvement of functional properties of bovine serum albumin through phosphorylation by dry-heating in the presence of pyrophosphate
Authors: Enomoto H, Li CP, Morizane K, Ibrahim HR, Sugimoto Y, Ohki S, Ohtomo H, Aoki T.
Journal: J Food Sci (2008): C84
Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio
Authors: Zheng C, Bi J, Ma G, Su Z.
Journal: Artif Cells Blood Substit Immobil Biotechnol (2007): 568
Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions
Authors: Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD.
Journal: Anal Bioanal Chem (2007): 969
Page updated on December 17, 2024

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Catalog Number5601
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Physical properties

Molecular weight

60000

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C)
UNSPSC12352200
Rabbits are immunized with BSA, BSA-AHD (Aminohydantoin), cBSA-AHD and hBSA-AHD conjugates. Antisera from immunized rabbits are tested for the immune response against AHD.&nbsp;&nbsp; Data indicate &nbsp;cBSA-AHD and hBSA-AHD exhibit a stronger immune response to AHD in comparison with BSA-AHD. Similar results are obtained for cOval and hOval conjugates (Mol Pharm. 2011 August 1; 8(4): 1174&ndash;1185).
Rabbits are immunized with BSA, BSA-AHD (Aminohydantoin), cBSA-AHD and hBSA-AHD conjugates. Antisera from immunized rabbits are tested for the immune response against AHD.&nbsp;&nbsp; Data indicate &nbsp;cBSA-AHD and hBSA-AHD exhibit a stronger immune response to AHD in comparison with BSA-AHD. Similar results are obtained for cOval and hOval conjugates (Mol Pharm. 2011 August 1; 8(4): 1174&ndash;1185).
Rabbits are immunized with BSA, BSA-AHD (Aminohydantoin), cBSA-AHD and hBSA-AHD conjugates. Antisera from immunized rabbits are tested for the immune response against AHD.&nbsp;&nbsp; Data indicate &nbsp;cBSA-AHD and hBSA-AHD exhibit a stronger immune response to AHD in comparison with BSA-AHD. Similar results are obtained for cOval and hOval conjugates (Mol Pharm. 2011 August 1; 8(4): 1174&ndash;1185).