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Fluorescamine *CAS 38183-12-9*
Fluorescamine is a popular amine-fluorogenic reagent for determining protein concentrations in solutions and on gels. It is also used to analyze low molecular weight amines by TLC, HPLC and capillary electrophoresis. Fluorescamine is nonfluorescent but readily reacts with primary aliphatic amines, including those in peptides and proteins, to yield a strongly blue-green fluorescent adduct that can be excited by UV light. The amine adduct has absorption maximum at 385 nm and fluorescence maximum at 486 nm.
Example protocol
AT A GLANCE
Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.
It can be used within 12 months from the date of receipt.
PREPARATION OF WORKING SOLUTION
Fluorescamine working solution:
Add anhydrous DMF or DMSO to make Fluorescamine working solution of desired concentration. Note: Fluorescamine reacts with water and many other aqueous buffers. It is recommended not to make buffer solutions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescamine *CAS 38183-12-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 359.376 µL | 1.797 mL | 3.594 mL | 17.969 mL | 35.938 mL |
| 5 mM | 71.875 µL | 359.376 µL | 718.752 µL | 3.594 mL | 7.188 mL |
| 10 mM | 35.938 µL | 179.688 µL | 359.376 µL | 1.797 mL | 3.594 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 41 references: Citation Explorer
Fluorescence of aromatic amines and their fluorescamine derivatives for detection of explosive vapors
Authors: Eastwood D, Fern and ez C, Yoon BY, Sheaff CN, Wai CM.
Journal: Appl Spectrosc (2006): 958
Authors: Eastwood D, Fern and ez C, Yoon BY, Sheaff CN, Wai CM.
Journal: Appl Spectrosc (2006): 958
Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent
Authors: Adamou R, Coly A, Douabale SE, Saleck ML, Gaye-Seye MD, Tine A.
Journal: J Fluoresc (2005): 679
Authors: Adamou R, Coly A, Douabale SE, Saleck ML, Gaye-Seye MD, Tine A.
Journal: J Fluoresc (2005): 679
Determination of 4-amino-m-cresol and 5-amino-o-cresol by high performance liquid chromatography and fluorescence derivatization using fluorescamine
Authors: Eggenreich K, Zach E, Beck H, Wintersteiger R.
Journal: J Biochem Biophys Methods (2004): 35
Authors: Eggenreich K, Zach E, Beck H, Wintersteiger R.
Journal: J Biochem Biophys Methods (2004): 35
Chiral separation of fluorescamine-labeled amino acids using microfabricated capillary electrophoresis devices for extraterrestrial exploration
Authors: Skelley AM, Mathies RA.
Journal: J Chromatogr A (2003): 191
Authors: Skelley AM, Mathies RA.
Journal: J Chromatogr A (2003): 191
LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations
Authors: Cesur N, Apak TI, Aboul-Enein HY, Ozkirimli S.
Journal: J Pharm Biomed Anal (2002): 487
Authors: Cesur N, Apak TI, Aboul-Enein HY, Ozkirimli S.
Journal: J Pharm Biomed Anal (2002): 487
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