Fluo-8L™, sodium salt
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Dissociation constant (Kd, nM) | 1900 |
Molecular weight | 828.54 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 1.076 |
Correction Factor (280 nm) | 0.769 |
Extinction coefficient (cm -1 M -1) | 23430 |
Excitation (nm) | 495 |
Emission (nm) | 516 |
Quantum yield | 0.161 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
Molecular weight 828.54 | Dissociation constant (Kd, nM) 1900 | Correction Factor (260 nm) 1.076 | Correction Factor (280 nm) 0.769 | Extinction coefficient (cm -1 M -1) 23430 | Excitation (nm) 495 | Emission (nm) 516 | Quantum yield 0.161 |
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluo-8L™, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 120.694 µL | 603.471 µL | 1.207 mL | 6.035 mL | 12.069 mL |
5 mM | 24.139 µL | 120.694 µL | 241.388 µL | 1.207 mL | 2.414 mL |
10 mM | 12.069 µL | 60.347 µL | 120.694 µL | 603.471 µL | 1.207 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluo_8.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluo_8.png&w=2048&q=50)
Spectral properties
Correction Factor (260 nm) | 1.076 |
Correction Factor (280 nm) | 0.769 |
Extinction coefficient (cm -1 M -1) | 23430 |
Excitation (nm) | 495 |
Emission (nm) | 516 |
Quantum yield | 0.161 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Fluo-8®, sodium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Fluo-8H™, sodium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Fluo-8L™, potassium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Citations
View all 153 citations: Citation Explorer
Human lysophosphatidic acid receptor 2-K1. 31 and K7. 36 gatekeeper functions provide new insight into robust affinity for LPA-type agonists.
Authors: Omotuyi, Olaposi I
Journal: Journal of Systems Biology & Proteome Research (2017)
Authors: Omotuyi, Olaposi I
Journal: Journal of Systems Biology & Proteome Research (2017)
Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Non-steroidal anti-inflammatory drug delays corneal wound healing by reducing production of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B4 receptor 2
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks
Authors: Ishii, Masaaki and Rohrer, Bärbel
Journal: Cell Death Discovery (2017): 16071
Authors: Ishii, Masaaki and Rohrer, Bärbel
Journal: Cell Death Discovery (2017): 16071
High Glucose Enhances Isoflurane-Induced Neurotoxicity by Regulating TRPC-Dependent Calcium Influx
Authors: Liu, ZhongJie and Ma, ChangQing and Zhao, Wei and Zhang, QingGuo and Xu, Rui and Zhang, HongFei and Lei, HongYi and Xu, ShiYuan
Journal: Neurochemical Research (2017): 1--14
Authors: Liu, ZhongJie and Ma, ChangQing and Zhao, Wei and Zhang, QingGuo and Xu, Rui and Zhang, HongFei and Lei, HongYi and Xu, ShiYuan
Journal: Neurochemical Research (2017): 1--14
Hyperforin/HP-β-Cyclodextrin Enhances Mechanosensitive Ca2
Authors: Takada, Hiroya and Yonekawa, Jun and Matsumoto, Masami and Furuya, Kishio and Sokabe, Masahiro
Journal: BioMed Research International (2017)
Authors: Takada, Hiroya and Yonekawa, Jun and Matsumoto, Masami and Furuya, Kishio and Sokabe, Masahiro
Journal: BioMed Research International (2017)
High-throughput screen detects calcium signaling dysfunction in typical sporadic autism spectrum disorder
Authors: Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay
Journal: Scientific Reports (2017): 40740
Authors: Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay
Journal: Scientific Reports (2017): 40740
Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Protective effect of quercetin on bupivacaine-induced neurotoxicity via T-type calcium channel inhibition
Authors: Jin, Chao and Wu, Huisheng and Tang, Chaoliang and Ke, Jianjuan and Wang, Yanlin
Journal: Tropical Journal of Pharmaceutical Research (2017): 1827--1833
Authors: Jin, Chao and Wu, Huisheng and Tang, Chaoliang and Ke, Jianjuan and Wang, Yanlin
Journal: Tropical Journal of Pharmaceutical Research (2017): 1827--1833
The establishment of appropriate methods for egg-activation by human PLCZ1 RNA injection into human oocyte
Authors: Yamaguchi, Takashi and Ito, Masahiko and Kuroda, Keiji and Takeda, Satoru and Tanaka, Atsushi
Journal: Cell Calcium (2017)
Authors: Yamaguchi, Takashi and Ito, Masahiko and Kuroda, Keiji and Takeda, Satoru and Tanaka, Atsushi
Journal: Cell Calcium (2017)
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?