Fluo-8L™, potassium salt
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Dissociation constant (Kd, nM) | 1900 |
Molecular weight | 909.09 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 1.076 |
Correction Factor (280 nm) | 0.769 |
Extinction coefficient (cm -1 M -1) | 23430 |
Excitation (nm) | 495 |
Emission (nm) | 516 |
Quantum yield | 0.161 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Fluoresceins
Molecular weight 909.09 | Dissociation constant (Kd, nM) 1900 | Correction Factor (260 nm) 1.076 | Correction Factor (280 nm) 0.769 | Extinction coefficient (cm -1 M -1) 23430 | Excitation (nm) 495 | Emission (nm) 516 | Quantum yield 0.161 |
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluo-8L™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 110 µL | 550.001 µL | 1.1 mL | 5.5 mL | 11 mL |
5 mM | 22 µL | 110 µL | 220 µL | 1.1 mL | 2.2 mL |
10 mM | 11 µL | 55 µL | 110 µL | 550.001 µL | 1.1 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluo_8.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluo_8.png&w=2048&q=50)
Spectral properties
Correction Factor (260 nm) | 1.076 |
Correction Factor (280 nm) | 0.769 |
Extinction coefficient (cm -1 M -1) | 23430 |
Excitation (nm) | 495 |
Emission (nm) | 516 |
Quantum yield | 0.161 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Fluo-8®, potassium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Fluo-8L™, sodium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Fluo-8FF™, potassium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Citations
View all 153 citations: Citation Explorer
Human lysophosphatidic acid receptor 2-K1. 31 and K7. 36 gatekeeper functions provide new insight into robust affinity for LPA-type agonists.
Authors: Omotuyi, Olaposi I
Journal: Journal of Systems Biology & Proteome Research (2017)
Authors: Omotuyi, Olaposi I
Journal: Journal of Systems Biology & Proteome Research (2017)
Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Non-steroidal anti-inflammatory drug delays corneal wound healing by reducing production of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B4 receptor 2
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks
Authors: Ishii, Masaaki and Rohrer, Bärbel
Journal: Cell Death Discovery (2017): 16071
Authors: Ishii, Masaaki and Rohrer, Bärbel
Journal: Cell Death Discovery (2017): 16071
High Glucose Enhances Isoflurane-Induced Neurotoxicity by Regulating TRPC-Dependent Calcium Influx
Authors: Liu, ZhongJie and Ma, ChangQing and Zhao, Wei and Zhang, QingGuo and Xu, Rui and Zhang, HongFei and Lei, HongYi and Xu, ShiYuan
Journal: Neurochemical Research (2017): 1--14
Authors: Liu, ZhongJie and Ma, ChangQing and Zhao, Wei and Zhang, QingGuo and Xu, Rui and Zhang, HongFei and Lei, HongYi and Xu, ShiYuan
Journal: Neurochemical Research (2017): 1--14
Hyperforin/HP-β-Cyclodextrin Enhances Mechanosensitive Ca2
Authors: Takada, Hiroya and Yonekawa, Jun and Matsumoto, Masami and Furuya, Kishio and Sokabe, Masahiro
Journal: BioMed Research International (2017)
Authors: Takada, Hiroya and Yonekawa, Jun and Matsumoto, Masami and Furuya, Kishio and Sokabe, Masahiro
Journal: BioMed Research International (2017)
High-throughput screen detects calcium signaling dysfunction in typical sporadic autism spectrum disorder
Authors: Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay
Journal: Scientific Reports (2017): 40740
Authors: Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay
Journal: Scientific Reports (2017): 40740
Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Protective effect of quercetin on bupivacaine-induced neurotoxicity via T-type calcium channel inhibition
Authors: Jin, Chao and Wu, Huisheng and Tang, Chaoliang and Ke, Jianjuan and Wang, Yanlin
Journal: Tropical Journal of Pharmaceutical Research (2017): 1827--1833
Authors: Jin, Chao and Wu, Huisheng and Tang, Chaoliang and Ke, Jianjuan and Wang, Yanlin
Journal: Tropical Journal of Pharmaceutical Research (2017): 1827--1833
The establishment of appropriate methods for egg-activation by human PLCZ1 RNA injection into human oocyte
Authors: Yamaguchi, Takashi and Ito, Masahiko and Kuroda, Keiji and Takeda, Satoru and Tanaka, Atsushi
Journal: Cell Calcium (2017)
Authors: Yamaguchi, Takashi and Ito, Masahiko and Kuroda, Keiji and Takeda, Satoru and Tanaka, Atsushi
Journal: Cell Calcium (2017)
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets