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FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK
Activation of caspases plays a central role in apoptosis. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. Based on our in-house studies FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK gave the highest signal/background ratio compared to other DEVD-FMK probes we tested. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 96.426 µL | 482.128 µL | 964.255 µL | 4.821 mL | 9.643 mL |
| 5 mM | 19.285 µL | 96.426 µL | 192.851 µL | 964.255 µL | 1.929 mL |
| 10 mM | 9.643 µL | 48.213 µL | 96.426 µL | 482.128 µL | 964.255 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
References
View all 4 references: Citation Explorer
Antitumor mechanism of 3-bromopropionylamino benzoylurea on leukemia and lymphoma
Authors: Li JN, Song DQ, Jiang JD.
Journal: Yao Xue Xue Bao (2004): 491
Authors: Li JN, Song DQ, Jiang JD.
Journal: Yao Xue Xue Bao (2004): 491
Activation of caspase-like activity and poly (ADP-ribose) polymerase degradation during sporulation in Aspergillus nidulans
Authors: Thrane C, Kaufmann U, Stummann BM, Olsson S.
Journal: Fungal Genet Biol (2004): 361
Authors: Thrane C, Kaufmann U, Stummann BM, Olsson S.
Journal: Fungal Genet Biol (2004): 361
Caspase-dependent and independent cell death in rat hepatoma 5123tc cells
Authors: P, undefined and ey S, Smith B, Walker PR, Sikorska M.
Journal: Apoptosis (2000): 265
Authors: P, undefined and ey S, Smith B, Walker PR, Sikorska M.
Journal: Apoptosis (2000): 265
C-myc antisense oligodeoxynucleotides can induce apoptosis and down-regulate Fas expression in rheumatoid synoviocytes
Authors: Hashiramoto A, Sano H, Maekawa T, Kawahito Y, Kimura S, Kusaka Y, Wilder RL, Kato H, Kondo M, Nakajima H.
Journal: Arthritis Rheum (1999): 954
Authors: Hashiramoto A, Sano H, Maekawa T, Kawahito Y, Kimura S, Kusaka Y, Wilder RL, Kato H, Kondo M, Nakajima H.
Journal: Arthritis Rheum (1999): 954
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