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EDTA, AM ester
Ethylenediaminetetraacetic acid (EDTA) might be the most popular metal ion chelator used for both industrial and medical purposes. It is widely used to chelate transitional metal ions, e.g., nickel, cobalt, ferric, ferrous, mercury, lead and other metal ions such as calcium and magnesium. After being bound by EDTA into a metal complex, metal ions generally exhibit diminished reactivity. EDTA is not permeable to live cells. EDTA, AM ester is the cell-permeant derivative of EDTA. Live cells are passively loaded with EDTA, AM by incubation with the cell membrane-permeant EDTA AM ester. Once internalized, cytosolic esterases cleave the AM esters to liberate the active tetra-carboxylate ligand EDTA that sequesters intracellular metal ions indiscriminately. For example, it can be used for sequestering cytosolic Ca2+ for investigations of the role of cytosolic calcium ion, or it can be used to sequester heavy metal contaminants or to inhibit metalloenzymes and metalloproteases that require divalent cations for activity.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EDTA, AM ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 172.265 µL | 861.326 µL | 1.723 mL | 8.613 mL | 17.227 mL |
| 5 mM | 34.453 µL | 172.265 µL | 344.531 µL | 1.723 mL | 3.445 mL |
| 10 mM | 17.227 µL | 86.133 µL | 172.265 µL | 861.326 µL | 1.723 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 8 citations: Citation Explorer
Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress
Authors: Madaris, Travis R and Venkatesan, Manigandan and Maity, Soumya and Stein, Miriam C and Vishnu, Neelanjan and Venkateswaran, Mridula K and Davis, James G and Ramachandran, Karthik and Uthayabalan, Sukanthathulse and Allen, Cristel and others,
Journal: Cell Reports (2023)
Authors: Madaris, Travis R and Venkatesan, Manigandan and Maity, Soumya and Stein, Miriam C and Vishnu, Neelanjan and Venkateswaran, Mridula K and Davis, James G and Ramachandran, Karthik and Uthayabalan, Sukanthathulse and Allen, Cristel and others,
Journal: Cell Reports (2023)
Effect of EDTA root conditioning on the outcome of coronally advanced flap with connective tissue graft: a systematic review and meta-analysis
Authors: Barootchi, S., Tavelli, L., Ravida, A., Wang, C. W., Wang, H. L.
Journal: Clin Oral Investig (2018): 2727-2741
Authors: Barootchi, S., Tavelli, L., Ravida, A., Wang, C. W., Wang, H. L.
Journal: Clin Oral Investig (2018): 2727-2741
Long-term risks of kidney living donation: review and position paper by the ERA-EDTA DESCARTES working group
Authors: Maggiore, U., Budde, K., Heemann, U., Hilbr and s, L., Oberbauer, R., Oniscu, G. C., Pascual, J., Schwartz Sorensen, S., Viklicky, O., Abramowicz, D., Era-Edta Descartes working group
Journal: Nephrol Dial Transplant (2017): 216-223
Authors: Maggiore, U., Budde, K., Heemann, U., Hilbr and s, L., Oberbauer, R., Oniscu, G. C., Pascual, J., Schwartz Sorensen, S., Viklicky, O., Abramowicz, D., Era-Edta Descartes working group
Journal: Nephrol Dial Transplant (2017): 216-223
Expanding access to HIV viral load testing: a systematic review of RNA stability in EDTA tubes and PPT beyond current time and temperature thresholds
Authors: Bonner, K., Siemieniuk, R. A., Boozary, A., Roberts, T., Fajardo, E., Cohn, J.
Journal: PLoS One (2014): e113813
Authors: Bonner, K., Siemieniuk, R. A., Boozary, A., Roberts, T., Fajardo, E., Cohn, J.
Journal: PLoS One (2014): e113813
EDTA chelation reappraisal following new clinical trials and regular use in millions of patients: review of preliminary findings and risk/benefit assessment
Authors: Born, T., Kontoghiorghe, C. N., Spyrou, A., Kolnagou, A., Kontoghiorghes, G. J.
Journal: Toxicol Mech Methods (2013): 11-7
Authors: Born, T., Kontoghiorghe, C. N., Spyrou, A., Kolnagou, A., Kontoghiorghes, G. J.
Journal: Toxicol Mech Methods (2013): 11-7
Page updated on November 21, 2024
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